Advantage of Using Colonic Washouts for Blastocystis Detection in Colorectal Cancer Patients

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2014 Apr 9

PMID 24708637

Citations 15

Authors

Vinoth Kumarasamy

April Camilla Roslani

Kuppusamy Umah Rani

Suresh Kumar Govind

Affiliations

Soon will be listed here.

Abstract

Background: There have been previous studies associating microorganisms to cancer and with our recent findings of Blastocytsis antigen having a higher in vitro proliferation of cancer cells strengthens the suspicion. Collecting faecal samples alone to associate this parasite with cancer may not be accurate due to the phenomenon of irregular shedding and the possible treatment administrated to the cancer patients. Hence, this become the basis to search for an alternate method of sample collection. Colonic washout is an almost complete washed up material from colon and rectum which includes various microorganisms such as Blastocystis and other lodged material within the villi. The detection of parasite in colonic washouts will give a better reflection on the association between Blastocystis and CRC.

Methods: Blastocytsis detection was made by in vitro culture method using Jones' medium, formal ether concentration technique and conventional polymerase chain reaction (PCR) on faecal samples and colonic washouts of 204 CRC patients from colonoscopy procedure. Faecal samples and colonic washouts from 221 normal individuals served as control.

Results: We observed an increased detection of Blastocystis using colonic washouts (n = 53, 12.47%) than faecal samples (n = 26, 6.12%). Eleven faecal samples showed positive results for Blastocystis which were also found in colonic washouts using the PCR technique. This study for the first time showed a significant Blastocystis infection among CRC patients (n = 43, 21.08%) compared to the asymptomatic normal individuals (n = 22, 9.95%). Blastocystis subtype 3 infection was found to be significantly more prevalent (n = 26, 12.75%) compared to other subtypes namely subtype 1: n = 9 (4.41%), subtype 2: n = 1 (0.49%), subtype 5: n = 1 (0.49%) and mixed subtype: n = 6 (2.94%) among the CRC patients.

Conclusion: The study showed that colonic washouts provide a better alternative for Blastocystis detection in CRC patients compared to faecal samples as this prevents treatment regime and the phenomenon of irregular shedding from influencing the detection results obtained from faecal samples.

Citing Articles

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Systematic Review and Meta-Analysis: Epidemiology of Human spp. Infection in Malaysia.

Kumarasamy V, Rajamanikam A, Anbazhagan D, Atroosh W, Azzani M, Subramaniyan V Trop Med Infect Dis. 2023; 8(8).

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Molecular Identification, Subtypes Distribution, and Alleles Discrimination of sp., Isolated from Immunocompromised Subjects in Iran.

Mohammad Rahimi H, Karamati S, Nemati S, Mirjalali H, Zali M Iran J Parasitol. 2022; 17(2):184-193.

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Are Blastocystis hominis and Cryptosporidium spp. playing a positive role in colorectal cancer risk? A systematic review and meta-analysis.

Taghipour A, Rayatdoost E, Bairami A, Bahadory S, Abdoli A Infect Agent Cancer. 2022; 17(1):32.

PMID: 35715853 PMC: 9206311. DOI: 10.1186/s13027-022-00447-x.

Association of with colorectal cancer: A systematic review of and evidences.

Kumarasamy V, Atroosh W, Anbazhagan D, Abdalla M, Azzani M World J Gastrointest Oncol. 2022; 14(3):734-745.

PMID: 35321272 PMC: 8919012. DOI: 10.4251/wjgo.v14.i3.734.

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