Amniotic Mesenchymal Stem Cells Display Neurovascular Tropism and Aid in the Recovery of Injured Peripheral Nerves

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2014 Apr 9

PMID 24708439

Citations 17

Authors

Yongnan Li

Longzhe Guo

Hyun Sook Ahn

Moo Hyun Kim

Sung-Whan Kim

Affiliations

Soon will be listed here.

Abstract

Recently, we reported that human amniotic membrane-derived mesenchymal stem cells (AMMs) possess great angiogenic potential. In this study, we determined whether local injection of AMMs ameliorates peripheral neuropathy. AMMs were transplanted into injured sciatic nerves. AMM injection promoted significant recovery of motor nerve conduction velocity and voltage amplitude compared to human adipose-derived mesenchymal stem cells. AMM implantation also augmented blood perfusion and increased intraneural vascularity. Whole-mount fluorescent imaging analysis demonstrated that AMMs exhibited higher engraftment and endothelial incorporation abilities in the sciatic nerve. In addition, the higher expression of pro-angiogenic factors was detected in AMMs injected into the peripheral nerve. Therefore, these data provide novel therapeutic and mechanistic insights into stem cell biology, and AMM transplantation may represent an alternative therapeutic option for treating peripheral neuropathy.

Citing Articles

Advanced techniques and innovations in peripheral nerve repair: a comprehensive review for clinical and experimental reference.

Zou Y, Zhang G, Yang Y, Huang H, Li Z, Chen X Rev Neurosci. 2024; .

PMID: 39566026 DOI: 10.1515/revneuro-2024-0101.

Biological properties and surgical applications of the human amniotic membrane.

Munoz-Torres J, Martinez-Gonzalez S, Lozano-Lujan A, Martinez-Vazquez M, Velasco-Elizondo P, Garza-Veloz I Front Bioeng Biotechnol. 2023; 10:1067480.

PMID: 36698632 PMC: 9868191. DOI: 10.3389/fbioe.2022.1067480.

Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats.

Beigom Hejazian L, Akbarnejad Z, Moghani Ghoroghi F, Esmaeilzade B, Chaibakhsh S Basic Clin Neurosci. 2023; 13(1):57-70.

PMID: 36589026 PMC: 9790101. DOI: 10.32598/bcn.2021.2240.1.

Peripheral Nerve Regeneration Using Different Germ Layer-Derived Adult Stem Cells in the Past Decade.

Li Y, Kamei Y, Kambe M, Ebisawa K, Oishi M, Takanari K Behav Neurol. 2021; 2021:5586523.

PMID: 34539934 PMC: 8448597. DOI: 10.1155/2021/5586523.

Characteristics and Therapeutic Potential of Human Amnion-Derived Stem Cells.

Liu Q, Huang Q, Wu H, Zuo G, Gu H, Deng K Int J Mol Sci. 2021; 22(2).

PMID: 33478081 PMC: 7835733. DOI: 10.3390/ijms22020970.

References

Schratzberger P, Walter D, Rittig K, Bahlmann F, Pola R, Curry C . Reversal of experimental diabetic neuropathy by VEGF gene transfer. J Clin Invest. 2001; 107(9):1083-92. PMC: 209283. DOI: 10.1172/JCI12188. View

Hu J, Zhu Q, Liu X, Xu Y, Zhu J . Repair of extended peripheral nerve lesions in rhesus monkeys using acellular allogenic nerve grafts implanted with autologous mesenchymal stem cells. Exp Neurol. 2007; 204(2):658-66. DOI: 10.1016/j.expneurol.2006.11.018. View

Palmer T, Willhoite A, Gage F . Vascular niche for adult hippocampal neurogenesis. J Comp Neurol. 2000; 425(4):479-94. DOI: 10.1002/1096-9861(20001002)425:4<479::aid-cne2>3.0.co;2-3. View

Cheng F, Tai M, Sheu M, Chen C, Yang D, Su H . Enhancement of regeneration with glia cell line-derived neurotrophic factor-transduced human amniotic fluid mesenchymal stem cells after sciatic nerve crush injury. J Neurosurg. 2009; 112(4):868-79. DOI: 10.3171/2009.8.JNS09850. View

Kim S, Zhang H, Kim C, Kim J, Kim M . Amniotic mesenchymal stem cells with robust chemotactic properties are effective in the treatment of a myocardial infarction model. Int J Cardiol. 2012; 168(2):1062-9. DOI: 10.1016/j.ijcard.2012.11.003. View

Zhang Z, Zhang L, Jiang Q, Zhang R, Davies K, Powers C . VEGF enhances angiogenesis and promotes blood-brain barrier leakage in the ischemic brain. J Clin Invest. 2000; 106(7):829-38. PMC: 517814. DOI: 10.1172/JCI9369. View

Walsh S, Midha R . Use of stem cells to augment nerve injury repair. Neurosurgery. 2009; 65(4 Suppl):A80-6. DOI: 10.1227/01.NEU.0000335651.93926.2F. View

Kim S, Kim H, Cho H, Lee J, Levit R, Yoon Y . Human peripheral blood-derived CD31+ cells have robust angiogenic and vasculogenic properties and are effective for treating ischemic vascular disease. J Am Coll Cardiol. 2010; 56(7):593-607. PMC: 2917842. DOI: 10.1016/j.jacc.2010.01.070. View

Noshita N, Lewen A, Sugawara T, Chan P . Evidence of phosphorylation of Akt and neuronal survival after transient focal cerebral ischemia in mice. J Cereb Blood Flow Metab. 2001; 21(12):1442-50. DOI: 10.1097/00004647-200112000-00009. View

10.

Sekiguchi H, Ii M, Jujo K, Thorne T, Ito A, Klyachko E . Estradiol promotes neural stem cell differentiation into endothelial lineage and angiogenesis in injured peripheral nerve. Angiogenesis. 2012; 16(1):45-58. PMC: 3806485. DOI: 10.1007/s10456-012-9298-5. View

11.

Byun K, Kim S . Is stem cell-based therapy going on or out for cardiac disease?. Korean Circ J. 2009; 39(3):87-92. PMC: 2771799. DOI: 10.4070/kcj.2009.39.3.87. View

12.

Tohill M, Terenghi G . Stem-cell plasticity and therapy for injuries of the peripheral nervous system. Biotechnol Appl Biochem. 2004; 40(Pt 1):17-24. DOI: 10.1042/BA20030173. View

13.

Cai L, Johnstone B, Cook T, Tan J, Fishbein M, Chen P . IFATS collection: Human adipose tissue-derived stem cells induce angiogenesis and nerve sprouting following myocardial infarction, in conjunction with potent preservation of cardiac function. Stem Cells. 2008; 27(1):230-7. PMC: 2936459. DOI: 10.1634/stemcells.2008-0273. View

14.

Olson L . Regeneration in the adult central nervous system: experimental repair strategies. Nat Med. 1997; 3(12):1329-35. DOI: 10.1038/nm1297-1329. View

15.

Kusano K, Allendoerfer K, Munger W, Pola R, Bosch-Marce M, Kirchmair R . Sonic hedgehog induces arteriogenesis in diabetic vasa nervorum and restores function in diabetic neuropathy. Arterioscler Thromb Vasc Biol. 2004; 24(11):2102-7. DOI: 10.1161/01.ATV.0000144813.44650.75. View

16.

Hayashi T, Abe K, Itoyama Y . Reduction of ischemic damage by application of vascular endothelial growth factor in rat brain after transient ischemia. J Cereb Blood Flow Metab. 1998; 18(8):887-95. DOI: 10.1097/00004647-199808000-00009. View

17.

Terenghi G . Peripheral nerve regeneration and neurotrophic factors. J Anat. 1999; 194 ( Pt 1):1-14. PMC: 1467889. DOI: 10.1046/j.1469-7580.1999.19410001.x. View

18.

Cho H, Lee N, Lee J, Choi Y, Ii M, Wecker A . Role of host tissues for sustained humoral effects after endothelial progenitor cell transplantation into the ischemic heart. J Exp Med. 2007; 204(13):3257-69. PMC: 2150988. DOI: 10.1084/jem.20070166. View

19.

Akbar M, Calderon F, Wen Z, Kim H . Docosahexaenoic acid: a positive modulator of Akt signaling in neuronal survival. Proc Natl Acad Sci U S A. 2005; 102(31):10858-63. PMC: 1182431. DOI: 10.1073/pnas.0502903102. View

20.

Dudek H, Datta S, Franke T, Birnbaum M, Yao R, Cooper G . Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science. 1997; 275(5300):661-5. DOI: 10.1126/science.275.5300.661. View