A New Method for Yeast Phagocytosis Analysis by Flow Cytometry

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2014 Apr 8

PMID 24704495

Citations 11

Authors

Catarina Carneiro

Catarina Vaz

Joana Carvalho-Pereira

Celia Pais

Paula Sampaio

Affiliations

Soon will be listed here.

Abstract

Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.

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