Differential Regulation of Ferritin Subunits and Iron Transport Proteins: an Effect of Targeted Hepatic X-irradiation

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Apr 3

PMID 24693535

Citations 2

Authors

Naila Naz

Shakil Ahmad

Silke Cameron

Federico Moriconi

Margret Rave-Frank

Hans Christiansen

Clemens Friedrich Hess

Giuliano Ramadori

Ihtzaz A Malik

Affiliations

Soon will be listed here.

Abstract

The current study aimed to investigate radiation-induced regulation of iron proteins including ferritin subunits in rats. Rat livers were selectively irradiated in vivo at 25 Gy. This dose can be used to model radiation effects to the liver without inducing overt radiation-induced liver disease. Sham-irradiated rats served as controls. Isolated hepatocytes were irradiated at 8 Gy. Ferritin light polypeptide (FTL) was detectable in the serum of sham-irradiated rats with an increase after irradiation. Liver irradiation increased hepatic protein expression of both ferritin subunits. A rather early increase (3 h) was observed for hepatic TfR1 and Fpn-1 followed by a decrease at 12 h. The increase in TfR2 persisted over the observed time. Parallel to the elevation of AST levels, a significant increase (24 h) in hepatic iron content was measured. Complete blood count analysis showed a significant decrease in leukocyte number with an early increase in neutrophil granulocytes and a decrease in lymphocytes. In vitro, a significant increase in ferritin subunits at mRNA level was detected after irradiation which was further induced with a combination treatment of irradiation and acute phase cytokine. Irradiation can directly alter the expression of ferritin subunits and this response can be strongly influenced by radiation-induced proinflammatory cytokines. FTL can be used as a serum marker for early phase radiation-induced liver damage.

Citing Articles

Immunosuppressant-Induced Oxidative Stress and Iron: A Paradigm Shift from Systemic to Intrahepatic Abnormalities.

Akhtar T, Ali G, Sheikh N Oxid Med Cell Longev. 2020; 2020:8675275.

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The anti-TNF-α antibody infliximab inhibits the expression of fat-transporter-protein FAT/CD36 in a selective hepatic-radiation mouse model.

Martius G, Cameron S, Rave-Frank M, Hess C, Wolff H, Malik I Int J Mol Sci. 2015; 16(3):4682-97.

PMID: 25739082 PMC: 4394442. DOI: 10.3390/ijms16034682.

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