Rho GTPases at the Crossroad of Signaling Networks in Mammals: Impact of Rho-GTPases on Microtubule Organization and Dynamics

Overview

Journal Small GTPases

Specialties Biochemistry
Cell Biology

Date 2014 Apr 3

PMID 24691223

Citations 39

Authors

Jose Wojnacki

Gonzalo Quassollo

Maria-Paz Marzolo

Alfredo Caceres

Affiliations

Soon will be listed here.

Abstract

Microtubule (MT) organization and dynamics downstream of external cues is crucial for maintaining cellular architecture and the generation of cell asymmetries. In interphase cells RhoA, Rac, and Cdc42, conspicuous members of the family of small Rho GTPases, have major roles in modulating MT stability, and hence polarized cell behaviors. However, MTs are not mere targets of Rho GTPases, but also serve as signaling platforms coupling MT dynamics to Rho GTPase activation in a variety of cellular conditions. In this article, we review some of the key studies describing the reciprocal relationship between small Rho-GTPases and MTs during migration and polarization.

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References

Nishimura T, Yamaguchi T, Kato K, Yoshizawa M, Nabeshima Y, Ohno S . PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1. Nat Cell Biol. 2005; 7(3):270-7. DOI: 10.1038/ncb1227. View

Daub H, Gevaert K, Vandekerckhove J, Sobel A, Hall A . Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16. J Biol Chem. 2000; 276(3):1677-80. DOI: 10.1074/jbc.C000635200. View

Ridley A, Hall A . The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell. 1992; 70(3):389-99. DOI: 10.1016/0092-8674(92)90163-7. View

Etienne-Manneville S . Microtubules in cell migration. Annu Rev Cell Dev Biol. 2013; 29:471-99. DOI: 10.1146/annurev-cellbio-101011-155711. View

Manneville J, Jehanno M, Etienne-Manneville S . Dlg1 binds GKAP to control dynein association with microtubules, centrosome positioning, and cell polarity. J Cell Biol. 2010; 191(3):585-98. PMC: 3003329. DOI: 10.1083/jcb.201002151. View

Birukova A, Adyshev D, Gorshkov B, Bokoch G, Birukov K, Verin A . GEF-H1 is involved in agonist-induced human pulmonary endothelial barrier dysfunction. Am J Physiol Lung Cell Mol Physiol. 2005; 290(3):L540-8. DOI: 10.1152/ajplung.00259.2005. View

Kirschner M, Mitchison T . Beyond self-assembly: from microtubules to morphogenesis. Cell. 1986; 45(3):329-42. DOI: 10.1016/0092-8674(86)90318-1. View

Burbank K, Mitchison T . Microtubule dynamic instability. Curr Biol. 2006; 16(14):R516-7. DOI: 10.1016/j.cub.2006.06.044. View

Janke C, Bulinski J . Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions. Nat Rev Mol Cell Biol. 2011; 12(12):773-86. DOI: 10.1038/nrm3227. View

10.

Henriquez D, Bodaleo F, Montenegro-Venegas C, Gonzalez-Billault C . The light chain 1 subunit of the microtubule-associated protein 1B (MAP1B) is responsible for Tiam1 binding and Rac1 activation in neuronal cells. PLoS One. 2013; 7(12):e53123. PMC: 3531375. DOI: 10.1371/journal.pone.0053123. View

11.

Fukuhara S, Chikumi H, Gutkind J . Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G(12) family to Rho. FEBS Lett. 2000; 485(2-3):183-8. DOI: 10.1016/s0014-5793(00)02224-9. View

12.

Wallar B, Alberts A . The formins: active scaffolds that remodel the cytoskeleton. Trends Cell Biol. 2003; 13(8):435-46. DOI: 10.1016/s0962-8924(03)00153-3. View

13.

Palazzo A, Eng C, Schlaepfer D, Marcantonio E, Gundersen G . Localized stabilization of microtubules by integrin- and FAK-facilitated Rho signaling. Science. 2004; 303(5659):836-9. DOI: 10.1126/science.1091325. View

14.

Conde C, Arias C, Robin M, Li A, Saito M, Chuang J . Evidence for the involvement of Lfc and Tctex-1 in axon formation. J Neurosci. 2010; 30(19):6793-800. PMC: 3845415. DOI: 10.1523/JNEUROSCI.5420-09.2010. View

15.

Breitsprecher D, Goode B . Formins at a glance. J Cell Sci. 2013; 126(Pt 1):1-7. PMC: 3603506. DOI: 10.1242/jcs.107250. View

16.

Dent E, Gupton S, Gertler F . The growth cone cytoskeleton in axon outgrowth and guidance. Cold Spring Harb Perspect Biol. 2010; 3(3). PMC: 3039926. DOI: 10.1101/cshperspect.a001800. View

17.

Nogales E, Wolf S, Downing K . Structure of the alpha beta tubulin dimer by electron crystallography. Nature. 1998; 391(6663):199-203. DOI: 10.1038/34465. View

18.

Goulimari P, Kitzing T, Knieling H, Brandt D, Offermanns S, Grosse R . Galpha12/13 is essential for directed cell migration and localized Rho-Dia1 function. J Biol Chem. 2005; 280(51):42242-51. DOI: 10.1074/jbc.M508690200. View

19.

Xiang S, Dusaban S, Brown J . Lysophospholipid receptor activation of RhoA and lipid signaling pathways. Biochim Biophys Acta. 2012; 1831(1):213-22. PMC: 4076288. DOI: 10.1016/j.bbalip.2012.09.004. View

20.

Young K, Copeland J . Formins in cell signaling. Biochim Biophys Acta. 2008; 1803(2):183-90. DOI: 10.1016/j.bbamcr.2008.09.017. View