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Development of an Allele-specific PCR for Escherichia Coli B2 Sub-typing, a Rapid and Easy to Perform Substitute of Multilocus Sequence Typing

Overview
Specialty Microbiology
Date 2014 Apr 2
PMID 24685601
Citations 40
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Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

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