Comparison of 16S RRNA Sequencing with Biochemical Testing for Species-level Identification of Clinical Isolates of Neisseria Spp

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2014 Mar 28

PMID 24671298

Citations 2

Authors

Arij Mechergui

Wafa Achour

Assia Ben Hassen

Affiliations

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Abstract

We aimed to compare accuracy of genus and species level identification of Neisseria spp. using biochemical testing and 16S rRNA sequence analysis. These methods were evaluated using 85 Neisseria spp. clinical isolates initially identified to the genus level by conventional biochemical tests and API NH system (Bio-Mérieux(®)). In 34 % (29/85), more than one possibility was given by 16S rRNA sequence analysis. In 6 % (5/85), one of the possibilities offered by 16S rRNA gene sequencing, agreed with the result given by biochemical testing. In 4 % (3/85), the same species was given by both methods. 16S rRNA gene sequencing results did not correlate well with biochemical tests.

Citing Articles

Genotyping of commensal Neisseria spp strains by pulsed-field gel electrophoresis and 16S rRNA gene sequencing.

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Utility of 16S rDNA Sequencing for Identification of Rare Pathogenic Bacteria.

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