IGF-1 Gene Expression is Differentially Regulated by Estrogen Receptors α and β in Mouse Endometrial Stromal Cells and Ovarian Granulosa Cells

Overview

Journal J Reprod Dev

Specialties Biology
Reproductive Medicine

Date 2014 Mar 28

PMID 24670778

Citations 14

Authors

Yuki Ogo

Shusuke Taniuchi

Fumiya Ojima

Sayo Hayashi

Itsuo Murakami

Yuka Saito

Sakae Takeuchi

Toshiyuki Kudo

Sumio Takahashi

Affiliations

Soon will be listed here.

Abstract

Insulin-like growth factor 1 (IGF-1) is involved in regulations of reproductive functions in rats and mice. IGF-1 expression is regulated by estrogen in several reproductive organs including the uterus and ovary. Two types of estrogen receptor (ERα and ERβ) are expressed in mouse uteri and ovaries, and it is unclear whether they differently mediate IGF-1 gene transcription. To clarify the roles of ERα and ERβ, mouse endometrial stromal cells and ovarian granulosa cells were treated with ligands specific for individual estrogen receptors. In endometrial stromal cells, propyl-pyrazole-triol (PPT; ERα-selective agonist) increased Igf1 mRNA expression, which was suppressed by methyl-piperidino-pyrazole (MPP, ERα-selective antagonist), while diarylpropionitrile (DPN, ERβ-potency selective agonist) increased Igf1 mRNA expression, which was inhibited by MPP but not by 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-α]pyrimidin-3-yl]phenol (PHTPP; ERβ antagonist). PHTPP enhanced the DPN-induced increase in Igf1 mRNA expression. In ovarian granulosa cells, E2 and DPN decreased Igf1 mRNA expression, whereas PPT did not affect Igf1 mRNA levels. In these cells, PHTPP inhibited the DPN-induced decrease in Igf1 mRNA expression. These results suggest that ERα facilitates Igf1 transcription, whereas ERβ appears to inhibit Igf1 gene transcription in mouse endometrial stromal cells and ovarian granulosa cells.

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References

Murphy L, Murphy L, Friesen H . Estrogen induces insulin-like growth factor-I expression in the rat uterus. Mol Endocrinol. 1987; 1(7):445-50. DOI: 10.1210/mend-1-7-445. View

Liu M, Albanese C, Anderson C, Hilty K, Webb P, Uht R . Opposing action of estrogen receptors alpha and beta on cyclin D1 gene expression. J Biol Chem. 2002; 277(27):24353-60. DOI: 10.1074/jbc.M201829200. View

Compton D, Sheng S, Carlson K, Rebacz N, Lee I, Katzenellenbogen B . Pyrazolo[1,5-a]pyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activity. J Med Chem. 2004; 47(24):5872-93. DOI: 10.1021/jm049631k. View

Zhu L, Pollard J . Estradiol-17beta regulates mouse uterine epithelial cell proliferation through insulin-like growth factor 1 signaling. Proc Natl Acad Sci U S A. 2007; 104(40):15847-51. PMC: 2000402. DOI: 10.1073/pnas.0705749104. View

Adamo M, Lowe Jr W, LeRoith D, Roberts Jr C . Insulin-like growth factor I messenger ribonucleic acids with alternative 5'-untranslated regions are differentially expressed during development of the rat. Endocrinology. 1989; 124(6):2737-44. DOI: 10.1210/endo-124-6-2737. View

Shimatsu A, Rotwein P . Sequence of two rat insulin-like growth factor I mRNAs differing within the 5' untranslated region. Nucleic Acids Res. 1987; 15(17):7196. PMC: 306220. DOI: 10.1093/nar/15.17.7196. View

Sato T, Wang G, Hardy M, Kurita T, Cunha G, Cooke P . Role of systemic and local IGF-I in the effects of estrogen on growth and epithelial proliferation of mouse uterus. Endocrinology. 2002; 143(7):2673-9. DOI: 10.1210/endo.143.7.8878. View

Zhou J, Kumar T, Matzuk M, BONDY C . Insulin-like growth factor I regulates gonadotropin responsiveness in the murine ovary. Mol Endocrinol. 1998; 11(13):1924-33. DOI: 10.1210/mend.11.13.0032. View

Sar M, Welsch F . Differential expression of estrogen receptor-beta and estrogen receptor-alpha in the rat ovary. Endocrinology. 1999; 140(2):963-71. DOI: 10.1210/endo.140.2.6533. View

10.

Harris H . Estrogen receptor-beta: recent lessons from in vivo studies. Mol Endocrinol. 2006; 21(1):1-13. DOI: 10.1210/me.2005-0459. View

11.

Zhou H, Carlson K, Stossi F, Katzenellenbogen B, Katzenellenbogen J . Analogs of methyl-piperidinopyrazole (MPP): antiestrogens with estrogen receptor alpha selective activity. Bioorg Med Chem Lett. 2008; 19(1):108-10. PMC: 2711511. DOI: 10.1016/j.bmcl.2008.11.006. View

12.

Montgomery S, Shaw L, Pantelides N, Taggart M, Austin C . Acute effects of oestrogen receptor subtype-specific agonists on vascular contractility. Br J Pharmacol. 2003; 139(7):1249-53. PMC: 1573963. DOI: 10.1038/sj.bjp.0705368. View

13.

Cheng G, Weihua Z, Makinen S, Makela S, Saji S, Warner M . A role for the androgen receptor in follicular atresia of estrogen receptor beta knockout mouse ovary. Biol Reprod. 2001; 66(1):77-84. DOI: 10.1095/biolreprod66.1.77. View

14.

Bjornstrom L, Sjoberg M . Mechanisms of estrogen receptor signaling: convergence of genomic and nongenomic actions on target genes. Mol Endocrinol. 2005; 19(4):833-42. DOI: 10.1210/me.2004-0486. View

15.

Adashi E, Resnick C, Payne D, Rosenfeld R, Matsumoto T, Hunter M . The mouse intraovarian insulin-like growth factor I system: departures from the rat paradigm. Endocrinology. 1997; 138(9):3881-90. DOI: 10.1210/endo.138.9.5363. View

16.

Klotz D, Hewitt S, Korach K, DiAugustine R . Activation of a uterine insulin-like growth factor I signaling pathway by clinical and environmental estrogens: requirement of estrogen receptor-alpha. Endocrinology. 2000; 141(9):3430-9. DOI: 10.1210/endo.141.9.7649. View

17.

Kamai Y, Mikawa S, Endo K, Sakai H, Komano T . Regulation of insulin-like growth factor-I expression in mouse preadipocyte Ob1771 cells. J Biol Chem. 1996; 271(17):9883-6. DOI: 10.1074/jbc.271.17.9883. View

18.

Komatsu N, Maekawa T, Takeuchi S, Takahashi S . Epidermal growth factor and transforming growth factor-alpha stimulate the proliferation of mouse uterine stromal cells. Zoolog Sci. 2003; 20(5):639-45. DOI: 10.2108/zsj.20.639. View

19.

Heldring N, Pike A, Andersson S, Matthews J, Cheng G, Hartman J . Estrogen receptors: how do they signal and what are their targets. Physiol Rev. 2007; 87(3):905-31. DOI: 10.1152/physrev.00026.2006. View

20.

Couse J, Korach K . Estrogen receptor null mice: what have we learned and where will they lead us?. Endocr Rev. 1999; 20(3):358-417. DOI: 10.1210/edrv.20.3.0370. View