Lotus Japonicus SUNERGOS1 Encodes a Predicted Subunit A of a DNA Topoisomerase VI That is Required for Nodule Differentiation and Accommodation of Rhizobial Infection

Overview

Journal Plant J

Specialties Biology
Cell Biology
Molecular Biology

Date 2014 Mar 26

PMID 24661810

Citations 10

Authors

Hwi Joong Yoon

Md Shakhawat Hossain

Mark Held

Hongwei Hou

Marilyn Kehl

Alexandre Tromas

Shusei Sato

Satoshi Tabata

Stig Uggerhoj Andersen

Jens Stougaard

Loretta Ross

Krzysztof Szczyglowski

Affiliations

Soon will be listed here.

Abstract

A symbiotic mutant of Lotus japonicus, called sunergos1-1 (suner1-1), originated from a har1-1 suppressor screen. suner1-1 supports epidermal infection by Mesorhizobium loti and initiates cell divisions for organogenesis of nodule primordia. However, these processes appear to be temporarily stalled early during symbiotic interaction, leading to a low nodule number phenotype. This defect is ephemeral and near wild-type nodule numbers are reached by suner1-1 at a later point after infection. Using an approach that combined map-based cloning and next-generation sequencing we have identified the causative mutation and show that the suner1-1 phenotype is determined by a weak recessive allele, with the corresponding wild-type SUNER1 locus encoding a predicted subunit A of a DNA topoisomerase VI. Our data suggest that at least one function of SUNER1 during symbiosis is to participate in endoreduplication, which is an essential step during normal differentiation of functional, nitrogen-fixing nodules.

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