Increased 18F-FDG Uptake is Predictive of Rupture in a Novel Rat Abdominal Aortic Aneurysm Rupture Model

Overview

Journal Ann Surg

Specialty General Surgery

Date 2014 Mar 22

PMID 24651130

Citations 26

Authors

Sean J English

Morand R Piert

Jose A Diaz

David Gordon

Abhijit Ghosh

Louis G DAlecy

Steven E Whitesall

Ashish K Sharma

Elise P DeRoo

Tessa Watt

Gang Su

Peter K Henke

Jonathan L Eliason

Gorav Ailawadi

Gilbert R Upchurch Jr

Affiliations

Soon will be listed here.

Abstract

Objective: To determine whether F-fluorodeoxyglucose (F-FDG) micro-positron emission tomography (micro-PET) can predict abdominal aortic aneurysm (AAA) rupture.

Background: An infrarenal AAA model is needed to study inflammatory mechanisms that drive rupture. F-FDG PET can detect vascular inflammation in animal models and patients.

Methods: After exposing Sprague-Dawley rats to intra-aortic porcine pancreatic elastase (PPE) (12 U/mL), AAA rupture was induced by daily, subcutaneous, β-aminopropionitrile (BAPN, 300 mg/kg, N = 24) administration. Negative control AAA animals (N = 15) underwent daily saline subcutaneous injection after PPE exposure. BAPN-exposed animals that did not rupture served as positive controls [nonruptured AAA (NRAAA) 14d, N = 9]. Rupture was witnessed using radiotelemetry. Maximum standard uptakes for F-FDG micro-PET studies were determined. Aortic wall PAI-1, uPA, and tPA concentrations were determined by western blot analyses. Interleukin (IL)-1β, IL-6, IL-10, and MIP-2 were determined by Bio-Plex bead array. Neutrophil and macrophage populations per high-power field were quantified. Matrix metalloproteinase (MMP) activities were determined by zymography.

Results: When comparing ruptured AAA (RAAA) to NRAAA 14d animals, increased focal F-FDG uptakes were detected at subsequent sites of rupture (P = 0.03). PAI-1 expression was significantly less in RAAA tissue (P = 0.01), with comparable uPA and decreased tPA levels (P = 0.02). IL-1β (P = 0.04), IL-6 (P = 0.001), IL-10 (P = 0.04), and MIP-2 (P = 0.02) expression, neutrophil (P = 0.02) and macrophage presence (P = 0.002), and MMP9 (P < 0.0001) activity were increased in RAAA tissue.

Conclusions: With this AAA rupture model, increased prerupture F-FDG uptake on micro-PET imaging was associated with increased inflammation in the ruptured AAA wall. F-FDG PET imaging may be used to monitor inflammatory changes before AAA rupture.

Citing Articles

Ketosis prevents abdominal aortic aneurysm rupture through C-C chemokine receptor type 2 downregulation and enhanced extracellular matrix balance.

Sastriques-Dunlop S, Elizondo-Benedetto S, Arif B, Meade R, Zaghloul M, Luehmann H Sci Rep. 2024; 14(1):1438.

PMID: 38228786 PMC: 10791699. DOI: 10.1038/s41598-024-51996-7.

Novel insight of N-methyladenosine modified subtypes in abdominal aortic aneurysm.

Wang K, Kan Q, Ye Y, Qiu J, Huang L, Wu R Front Genet. 2022; 13:1055396.

PMID: 36482903 PMC: 9723249. DOI: 10.3389/fgene.2022.1055396.

Collagen cross-link profiles and mineral are different between the mandible and femur with site specific response to perturbed collagen.

Romanowicz G, Terhune A, Bielajew B, Sexton B, Lynch M, Mandair G Bone Rep. 2022; 17:101629.

PMID: 36325166 PMC: 9618783. DOI: 10.1016/j.bonr.2022.101629.

Identification of MEDAG and SERPINE1 Related to Hypoxia in Abdominal Aortic Aneurysm Based on Weighted Gene Coexpression Network Analysis.

Teng B, Xie C, Zhao Y, Zeng Q, Zhan F, Feng Y Front Physiol. 2022; 13:926508.

PMID: 35874515 PMC: 9301186. DOI: 10.3389/fphys.2022.926508.

Establishment of a New Abdominal Aortic Aneurysm Model in Rats by a Retroperitoneal Approach.

Zhu J, Tang Q, Zhou C, Shi X, Yi S, Yang Y Front Cardiovasc Med. 2022; 9:808732.

PMID: 35282381 PMC: 8905142. DOI: 10.3389/fcvm.2022.808732.

References

Kurihara T, Shimizu-Hirota R, Shimoda M, Adachi T, Shimizu H, Weiss S . Neutrophil-derived matrix metalloproteinase 9 triggers acute aortic dissection. Circulation. 2012; 126(25):3070-80. DOI: 10.1161/CIRCULATIONAHA.112.097097. View

Cho B, Woodrum D, Roelofs K, Stanley J, Henke P, Upchurch Jr G . Differential regulation of aortic growth in male and female rodents is associated with AAA development. J Surg Res. 2008; 155(2):330-8. PMC: 3205088. DOI: 10.1016/j.jss.2008.07.027. View

Kotze C, Menezes L, Endozo R, Groves A, Ell P, Yusuf S . Increased metabolic activity in abdominal aortic aneurysm detected by 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography/computed tomography (PET/CT). Eur J Vasc Endovasc Surg. 2009; 38(1):93-9. DOI: 10.1016/j.ejvs.2008.12.016. View

Knoess C, Siegel S, Smith A, Newport D, Richerzhagen N, Winkeler A . Performance evaluation of the microPET R4 PET scanner for rodents. Eur J Nucl Med Mol Imaging. 2003; 30(5):737-47. DOI: 10.1007/s00259-002-1052-6. View

Sakalihasan N, Damme H, Gomez P, Rigo P, Lapiere C, Nusgens B . Positron emission tomography (PET) evaluation of abdominal aortic aneurysm (AAA). Eur J Vasc Endovasc Surg. 2002; 23(5):431-6. DOI: 10.1053/ejvs.2002.1646. View

Thompson R, Curci J, Ennis T, Mao D, Pagano M, Pham C . Pathophysiology of abdominal aortic aneurysms: insights from the elastase-induced model in mice with different genetic backgrounds. Ann N Y Acad Sci. 2006; 1085:59-73. DOI: 10.1196/annals.1383.029. View

Cho B, Roelofs K, Ford J, Henke P, Upchurch Jr G . Decreased collagen and increased matrix metalloproteinase-13 in experimental abdominal aortic aneurysms in males compared with females. Surgery. 2009; 147(2):258-67. PMC: 3017342. DOI: 10.1016/j.surg.2009.06.047. View

Roderfeld M, Graf J, Giese B, Salguero-Palacios R, Tschuschner A, Muller-Newen G . Latent MMP-9 is bound to TIMP-1 before secretion. Biol Chem. 2007; 388(11):1227-34. DOI: 10.1515/BC.2007.123. View

Huffman M, Curci J, Moore G, Kerns D, Starcher B, Thompson R . Functional importance of connective tissue repair during the development of experimental abdominal aortic aneurysms. Surgery. 2000; 128(3):429-38. DOI: 10.1067/msy.2000.107379. View

10.

Lee S, Guo S, Scannevin R, Magliaro B, Rhodes K, Wang X . Induction of matrix metalloproteinase, cytokines and chemokines in rat cortical astrocytes exposed to plasminogen activators. Neurosci Lett. 2007; 417(1):1-5. DOI: 10.1016/j.neulet.2007.01.017. View

11.

Rateri D, Howatt D, Moorleghen J, Charnigo R, Cassis L, Daugherty A . Prolonged infusion of angiotensin II in apoE(-/-) mice promotes macrophage recruitment with continued expansion of abdominal aortic aneurysm. Am J Pathol. 2011; 179(3):1542-8. PMC: 3157213. DOI: 10.1016/j.ajpath.2011.05.049. View

12.

Visser E, Philippens M, Kienhorst L, Kaanders J, Corstens F, de Geus-Oei L . Comparison of tumor volumes derived from glucose metabolic rate maps and SUV maps in dynamic 18F-FDG PET. J Nucl Med. 2008; 49(6):892-8. DOI: 10.2967/jnumed.107.049585. View

13.

Sinha I, Cho B, Roelofs K, Stanley J, Henke P, Upchurch Jr G . Female gender attenuates cytokine and chemokine expression and leukocyte recruitment in experimental rodent abdominal aortic aneurysms. Ann N Y Acad Sci. 2006; 1085:367-79. DOI: 10.1196/annals.1383.027. View

14.

Sakalihasan N, Hustinx R, Limet R . Contribution of PET scanning to the evaluation of abdominal aortic aneurysm. Semin Vasc Surg. 2004; 17(2):144-53. DOI: 10.1053/j.semvascsurg.2004.03.002. View

15.

Patel R, Janoudi A, Vedre A, Aziz K, Tamhane U, Rubinstein J . Plaque rupture and thrombosis are reduced by lowering cholesterol levels and crystallization with ezetimibe and are correlated with fluorodeoxyglucose positron emission tomography. Arterioscler Thromb Vasc Biol. 2011; 31(9):2007-14. DOI: 10.1161/ATVBAHA.111.226167. View

16.

Sho E, Sho M, Nanjo H, Kawamura K, Masuda H, Dalman R . Comparison of cell-type-specific vs transmural aortic gene expression in experimental aneurysms. J Vasc Surg. 2005; 41(5):844-52. DOI: 10.1016/j.jvs.2005.02.027. View

17.

Killeen S, Neary P, OSullivan M, Redmond H, Fulton G . Daily diurnal variation in admissions for ruptured abdominal aortic aneurysms. World J Surg. 2007; 31(9):1869-1871. DOI: 10.1007/s00268-007-9126-z. View

18.

Booth N, Simpson A, Croll A, Bennett B, MacGREGOR I . Plasminogen activator inhibitor (PAI-1) in plasma and platelets. Br J Haematol. 1988; 70(3):327-33. DOI: 10.1111/j.1365-2141.1988.tb02490.x. View

19.

Wallinder J, Skagius E, Bergqvist D, Henriksson A . Early inflammatory response in patients with ruptured abdominal aortic aneurysm. Vasc Endovascular Surg. 2009; 44(1):32-5. DOI: 10.1177/1538574409339358. View

20.

Sinha I, Pearce C, Cho B, Hannawa K, Roelofs K, Stanley J . Differential regulation of the superoxide dismutase family in experimental aortic aneurysms and rat aortic explants. J Surg Res. 2007; 138(2):156-62. DOI: 10.1016/j.jss.2006.07.011. View