A Cell Culture Model of Chemically and Spontaneously Derived Mouse Lung Alveologenic Carcinoma

Overview

Journal Cell Biol Toxicol

Publisher Springer

Specialties Cell Biology
Toxicology

Date 1988 Sep 1

PMID 2465073

Citations 1

Authors

G J Smith

J G Steele

J M Bentel

C K Loo

Affiliations

Soon will be listed here.

Abstract

Malignant cell lines related to mouse lung alveologenic carcinoma have been established from urethane-induced tumors and after in vitro spontaneous transformation of preneoplastic cell lines. Both the chemically and spontaneously transformed cell lines formed invasive, poorly differentiated carcinomas with secondary lung deposits when implanted subcutaneously in immune-suppressed mice. They differed from the related preneoplastic cell line in coordinately exhibiting anchorage-independent growth, reduced epidermal growth factor receptor activity and absence of pericellular fibronectin. These data suggest that similar molecular events may occur in type 2 pneumocyte-related cells in order to generate mouse lung alveologenic adenomas and carcinomas by both spontaneous and chemical carcinogen induction mechanisms. A reduced level of pericellular fibronectin was also demonstrated in an in situ compressive urethane-induced mouse lung adenoma. Loss of pericellular fibronectin may therefore be an early and persistent phenotypic alteration during transformation to the alveologenic adenoma and carcinoma.

Citing Articles

Enhanced anchorage independent colony formation at increased agar concentrations in malignant mouse epithelial cell lines.

Bentel J, Smith G In Vitro Cell Dev Biol. 1990; 26(2):103-4.

PMID: 2312493 DOI: 10.1007/BF02624099.

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