Analysis of Essential Pathways for Self-renewal in Common Marmoset Embryonic Stem Cells

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2014 Mar 21

PMID 24649403

Citations 7

Authors

Takenobu Nii

Tomotoshi Marumoto

Hirotaka Kawano

Saori Yamaguchi

Jiyuan Liao

Michiyo Okada

Erika Sasaki

Yoshie Miura

Kenzaburo Tani

Affiliations

Soon will be listed here.

Abstract

Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.

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