Cyclooxygenase-2 Expression in Keratocystic Odontogenic Tumour Decreased Following Decompression

Overview

Journal Mol Clin Oncol

Specialty Oncology

Date 2014 Mar 21

PMID 24649281

Citations 2

Authors

Jing Wang

Xiaomin Zhang

Xu Ding

Shuzhong Xing

Huaiqi Li

Wei Zhang

Lizhen Wang

Heming Wu

Affiliations

Soon will be listed here.

Abstract

Marsupialisation or decompression is frequently performed as a conservative therapy for keratocystic odontogenic tumours (KCOTs). Positive cyclooxygenase-2 (COX-2) expression in the epithelium of KCOTs was recently reported and may be associated with neoplastic invasion and progression. The aim of the present study was to investigate the change in COX-2 expression in the KCOT epithelium following decompression. In this study, 16 pairs of KCOT biopsy specimens obtained during decompression or enucleation were collected and analysed. Formalin-fixed, paraffin-embedded blocks were sectioned and immunohistochemically investigated using anti-COX-2 antibody. The molecular expression was semi-quantitatively evaluated as follows: 0, negative; 1, weakly to moderately positive; and 2, strongly positive. In the samples obtained prior to decompression, the positive staining for COX-2 was immunolocalised to the cell membrane and the cytoplasm, it involved the full thickness of the epithelium and 15 of the 16 specimens (93.8%) exhibited mild to strong positivity. As regards the samples obtained following decompression, only 3 of the 16 specimens (18.8%) exhibited a mild positivity. The expression levels of COX-2 were significantly decreased following decompression (P<0.05). It may be concluded that loss or a significant reduction of COX-2 expression is associated with decompression in KCOTs. However, large-scale studies are required to verify these results and improve our knowledge of the possible involvement of COX-2 in the pathogenic mechanism underlying the development of KCOTs.

Citing Articles

Investigation of clinicopathological parameters and expression of COX-2, bcl-2, PCNA, and p53 in primary and recurrent sporadic odontogenic keratocysts.

Kaczmarzyk T, Kisielowski K, Koszowski R, Rynkiewicz M, Gawelek E, Babiuch K Clin Oral Investig. 2018; 22(9):3097-3106.

PMID: 29508125 PMC: 6224022. DOI: 10.1007/s00784-018-2400-7.

Giant keratocystic odontogenic tumor of the mandible - a case report.

Kornafel O, Jazwiec P, Pakulski K Pol J Radiol. 2015; 79:498-501.

PMID: 25566331 PMC: 4280059. DOI: 10.12659/PJR.892048.

References

Bhargava D, Deshpande A, Anthony Pogrel M . Keratocystic odontogenic tumour (KCOT)--a cyst to a tumour. Oral Maxillofac Surg. 2011; 16(2):163-70. DOI: 10.1007/s10006-011-0302-9. View

Kubota Y, Ninomiya T, Oka S, Takenoshita Y, Shirasuna K . Interleukin-1alpha-dependent regulation of matrix metalloproteinase-9(MMP-9) secretion and activation in the epithelial cells of odontogenic jaw cysts. J Dent Res. 2000; 79(6):1423-30. DOI: 10.1177/00220345000790061201. View

Kim D, Ahn S, Kim J, Yoon J . Comparative Ki-67 expression and apoptosis in the odontogenic keratocyst associated with or without an impacted tooth in addition to unilocular and multilocular varieties. Yonsei Med J. 2003; 44(5):841-6. DOI: 10.3349/ymj.2003.44.5.841. View

Mohan S, Epstein J . Carcinogenesis and cyclooxygenase: the potential role of COX-2 inhibition in upper aerodigestive tract cancer. Oral Oncol. 2003; 39(6):537-46. DOI: 10.1016/s1368-8375(03)00035-6. View

Nakamura N, Mitsuyasu T, Mitsuyasu Y, Taketomi T, Higuchi Y, Ohishi M . Marsupialization for odontogenic keratocysts: long-term follow-up analysis of the effects and changes in growth characteristics. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 94(5):543-53. DOI: 10.1067/moe.2002.128022. View

Williams C, Tsujii M, Reese J, Dey S, DuBois R . Host cyclooxygenase-2 modulates carcinoma growth. J Clin Invest. 2000; 105(11):1589-94. PMC: 300858. DOI: 10.1172/JCI9621. View

August M, Faquin W, Troulis M, Kaban L . Dedifferentiation of odontogenic keratocyst epithelium after cyst decompression. J Oral Maxillofac Surg. 2003; 61(6):678-83; discussion 683-4. DOI: 10.1053/joms.2003.50137. View

Suyama Y, Kubota Y, Yamashiro T, Ninomiya T, Koji T, Shirasuna K . Expression of keratinocyte growth factor and its receptor in odontogenic keratocysts. J Oral Pathol Med. 2009; 38(5):476-80. DOI: 10.1111/j.1600-0714.2009.00760.x. View

Madras J, Lapointe H . Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour. J Can Dent Assoc. 2008; 74(2):165-165h. View

10.

Suyama Y, Kubota Y, Ninomiya T, Shirasuna K . Immunohistochemical analysis of interleukin-1 alpha, its type I receptor and antagonist in keratocystic odontogenic tumors. J Oral Pathol Med. 2008; 37(9):560-4. DOI: 10.1111/j.1600-0714.2008.00667.x. View

11.

Piattelli A, Fioroni M, Santinelli A, Rubini C . Expression of proliferating cell nuclear antigen in ameloblastomas and odontogenic cysts. Oral Oncol. 1998; 34(5):408-12. DOI: 10.1016/s1368-8375(98)00027-x. View

12.

Shudou H, Sasaki M, Yamashiro T, Tsunomachi S, Takenoshita Y, Kubota Y . Marsupialisation for keratocystic odontogenic tumours in the mandible: longitudinal image analysis of tumour size using 3D visualised CT scans. Int J Oral Maxillofac Surg. 2011; 41(3):290-6. DOI: 10.1016/j.ijom.2011.10.015. View

13.

Li T, BROWNE R, Prime S, Paterson I, Matthews J . p53 expression in odontogenic keratocyst epithelium. J Oral Pathol Med. 1996; 25(5):249-55. DOI: 10.1111/j.1600-0714.1996.tb01380.x. View

14.

Kanzaki H, Chiba M, Shimizu Y, Mitani H . Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J Bone Miner Res. 2002; 17(2):210-20. DOI: 10.1359/jbmr.2002.17.2.210. View

15.

Anthony Pogrel M, Jordan R . Marsupialization as a definitive treatment for the odontogenic keratocyst. J Oral Maxillofac Surg. 2004; 62(6):651-5. DOI: 10.1016/j.joms.2003.08.029. View

16.

Ninomiya T, Kubota Y, Koji T, Shirasuna K . Marsupialization inhibits interleukin-1alpha expression and epithelial cell proliferation in odontogenic keratocysts. J Oral Pathol Med. 2002; 31(9):526-33. DOI: 10.1034/j.1600-0714.2002.00029.x. View

17.

Amaral Mendes R, Carvalho J, van der Waal I . A comparative immunohistochemical analysis of COX-2, p53, and Ki-67 expression in keratocystic odontogenic tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011; 111(3):333-9. DOI: 10.1016/j.tripleo.2010.10.004. View

18.

Gallo O, Masini E, Bianchi B, Bruschini L, Paglierani M, Franchi A . Prognostic significance of cyclooxygenase-2 pathway and angiogenesis in head and neck squamous cell carcinoma. Hum Pathol. 2002; 33(7):708-14. DOI: 10.1053/hupa.2002.125376. View

19.

Shear M . The aggressive nature of the odontogenic keratocyst: is it a benign cystic neoplasm? Part 2. Proliferation and genetic studies. Oral Oncol. 2002; 38(4):323-31. DOI: 10.1016/s1368-8375(01)00066-5. View

20.

Amaral Mendes R, Carvalho J, van der Waal I . An overview on the expression of cyclooxygenase-2 in tumors of the head and neck. Oral Oncol. 2009; 45(10):e124-8. DOI: 10.1016/j.oraloncology.2009.03.016. View