Expression of Sfrp2 is Increased in Catagen of Hair Follicles and Inhibits Keratinocyte Proliferation

Overview

Journal Ann Dermatol

Specialty Dermatology

Date 2014 Mar 21

PMID 24648690

Citations 13

Authors

Bong-Kyu Kim

Sungjoo Kim Yoon

Affiliations

Soon will be listed here.

Abstract

Background: Hair follicles undergo cycles of repeated growth and regression. The Wnt pathway plays an important role in the regeneration and differentiation of hair follicles. Sfrp2, a Wnt inhibitor, is involved in the developmental and disease processes of various cells and tissues by modulating the Wnt pathway.

Objective: The aim of this study was to understand the role of Sfrp2 in hair follicles through investigation of the Sfrp2 expression pattern in the skin and its effect on keratinocytes.

Methods: We investigated Sfrp2 mRNA expression and the expression of the wnt target genes, Ccnd1 and C-myc, at various mouse hair follicle developmental stages using Real-time polymerase chain reaction. We also investigated the effect of SFRP2 on the proliferation and differentiation of mouse keratinocyte cells by adding SFRP2 protein or overexpressing Sfrp2 using an in vitro culture system.

Results: Sfrp2 expression peaked in the catagen phase and remained high until telogen, and then declined at the beginning of the next anagen. An inverse relationship to Sfrp2 expression was found for the expression of the Wnt target genes, C-myc and Ccnd1. In addition, we also observed inhibited proliferation of mouse keratinocytes in the presence of SFRP2.

Conclusion: These results suggest that Sfrp2 may play a role in the catagen phase by inhibiting the proliferation of keratinocyte and functioning as a Wnt inhibitor in keratinocytes.

Citing Articles

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Hair follicle-resident progenitor cells are a major cellular contributor to heterotopic subcutaneous ossifications in a mouse model of Albright hereditary osteodystrophy.

McMullan P, Maye P, Root S, Yang Q, Edie S, Rowe D bioRxiv. 2024; .

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Comprehensive transcriptome profiling between balding and non-balding scalp of female pattern hair loss in Asian.

Kim S, Kim Y, Kim J, Kim S, Park W, Kim S Arch Dermatol Res. 2024; 316(7):360.

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Melatonin promotes hair regeneration by modulating the Wnt/β-catenin signalling pathway.

Niu Y, Li Y, Gao C, Li W, Li L, Wang H Cell Prolif. 2024; 57(9):e13656.

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Single nucleotide polymorphisms in the KRT82 promoter region modulate irregular thickening and patchiness in the dorsal skin of New Zealand rabbits.

Zhao B, Cai J, Zhang X, Li J, Bao Z, Chen Y BMC Genomics. 2024; 25(1):458.

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References

Ezan J, Leroux L, Barandon L, Dufourcq P, Jaspard B, Moreau C . FrzA/sFRP-1, a secreted antagonist of the Wnt-Frizzled pathway, controls vascular cell proliferation in vitro and in vivo. Cardiovasc Res. 2004; 63(4):731-8. DOI: 10.1016/j.cardiores.2004.05.006. View

Rutberg S, Kolpak M, Gourley J, Tan G, Henry J, Shander D . Differences in expression of specific biomarkers distinguish human beard from scalp dermal papilla cells. J Invest Dermatol. 2006; 126(12):2583-95. DOI: 10.1038/sj.jid.5700454. View

Hoang B, Moos Jr M, Vukicevic S, Luyten F . Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis. J Biol Chem. 1996; 271(42):26131-7. DOI: 10.1074/jbc.271.42.26131. View

Andl T, Reddy S, Gaddapara T, Millar S . WNT signals are required for the initiation of hair follicle development. Dev Cell. 2002; 2(5):643-53. DOI: 10.1016/s1534-5807(02)00167-3. View

Maganga R, Giles N, Adcroft K, Unni A, Keeney D, Wood F . Secreted Frizzled related protein-4 (sFRP4) promotes epidermal differentiation and apoptosis. Biochem Biophys Res Commun. 2008; 377(2):606-611. DOI: 10.1016/j.bbrc.2008.10.050. View

Baek I, Kim J, Cho K, Cha D, Cho J, Park J . A novel mutation in Hr causes abnormal hair follicle morphogenesis in hairpoor mouse, an animal model for Marie Unna Hereditary Hypotrichosis. Mamm Genome. 2009; 20(6):350-8. DOI: 10.1007/s00335-009-9191-8. View

Sick S, Reinker S, Timmer J, Schlake T . WNT and DKK determine hair follicle spacing through a reaction-diffusion mechanism. Science. 2006; 314(5804):1447-50. DOI: 10.1126/science.1130088. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Nelson W, Nusse R . Convergence of Wnt, beta-catenin, and cadherin pathways. Science. 2004; 303(5663):1483-7. PMC: 3372896. DOI: 10.1126/science.1094291. View

10.

Alfaro M, Pagni M, Vincent A, Atkinson J, Hill M, Cates J . The Wnt modulator sFRP2 enhances mesenchymal stem cell engraftment, granulation tissue formation and myocardial repair. Proc Natl Acad Sci U S A. 2008; 105(47):18366-71. PMC: 2587631. DOI: 10.1073/pnas.0803437105. View

11.

Kawano Y, Kypta R . Secreted antagonists of the Wnt signalling pathway. J Cell Sci. 2003; 116(Pt 13):2627-34. DOI: 10.1242/jcs.00623. View

12.

Chang C, Jiang T, Lin C, Burrus L, Chuong C, Widelitz R . Distinct Wnt members regulate the hierarchical morphogenesis of skin regions (spinal tract) and individual feathers. Mech Dev. 2004; 121(2):157-71. PMC: 4376312. DOI: 10.1016/j.mod.2003.12.004. View

13.

Ladher R, Church V, Allen S, Robson L, Abdelfattah A, Brown N . Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development. Dev Biol. 2000; 218(2):183-98. DOI: 10.1006/dbio.1999.9586. View

14.

Satoh W, Gotoh T, Tsunematsu Y, Aizawa S, Shimono A . Sfrp1 and Sfrp2 regulate anteroposterior axis elongation and somite segmentation during mouse embryogenesis. Development. 2006; 133(6):989-99. DOI: 10.1242/dev.02274. View

15.

Magerl M, Tobin D, Muller-Rover S, Hagen E, Lindner G, McKay I . Patterns of proliferation and apoptosis during murine hair follicle morphogenesis. J Invest Dermatol. 2001; 116(6):947-55. DOI: 10.1046/j.0022-202x.2001.01368.x. View

16.

Lee J, Chang C, Wu S, Sargan D, Lin C . Secreted frizzled-related protein 2 (SFRP2) is highly expressed in canine mammary gland tumors but not in normal mammary glands. Breast Cancer Res Treat. 2004; 84(2):139-49. DOI: 10.1023/B:BREA.0000018412.83348.ff. View

17.

Millar S, Willert K, Salinas P, Roelink H, Nusse R, Sussman D . WNT signaling in the control of hair growth and structure. Dev Biol. 1999; 207(1):133-49. DOI: 10.1006/dbio.1998.9140. View

18.

Schmidt-Ullrich R, Paus R . Molecular principles of hair follicle induction and morphogenesis. Bioessays. 2005; 27(3):247-61. DOI: 10.1002/bies.20184. View

19.

Kress E, Rezza A, Nadjar J, Samarut J, Plateroti M . The frizzled-related sFRP2 gene is a target of thyroid hormone receptor alpha1 and activates beta-catenin signaling in mouse intestine. J Biol Chem. 2008; 284(2):1234-41. DOI: 10.1074/jbc.M806548200. View

20.

Alonso L, Fuchs E . Stem cells in the skin: waste not, Wnt not. Genes Dev. 2003; 17(10):1189-200. DOI: 10.1101/gad.1086903. View