Crystallization and Preliminary X-ray Diffraction Analysis of Human DNA Primase

Overview

Journal Acta Crystallogr F Struct Biol Commun

Specialty Chemistry

Date 2014 Mar 19

PMID 24637758

Citations 5

Authors

Andrey G Baranovskiy

Jianyou Gu

Nigar D Babayeva

Vinod B Agarkar

Yoshiaki Suwa

Tahir H Tahirov

Affiliations

Soon will be listed here.

Abstract

Human primase synthesizes RNA primers and transfers them to the active site of Pol α with subsequent extension with dNTPs. Human primase is a heterodimer of two subunits: a small catalytic subunit (p49) and a large subunit (p58). The structural details of the initiation and elongation steps of primer synthesis, as well as primer length counting, are not known. To address these questions, structural studies of human primase were initiated. Two types of crystals were obtained. The best diffracting crystals belonged to space group P1, with unit-cell parameters a = 86.2, b = 88.9, c = 94.68 Å, α = 93.82, β = 96.57, γ = 111.72°, and contained two heterodimers of full-length p49 and p59 subunits in the asymmetric unit.

Citing Articles

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Mechanism of Concerted RNA-DNA Primer Synthesis by the Human Primosome.

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Crystal structure of the human primase.

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The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the human DNA polymerase α-primase complex.

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