Differential Regulation of CASZ1 Protein Expression During Cardiac and Skeletal Muscle Development

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2014 Mar 18

PMID 24633745

Citations 12

Authors

Nirav M Amin

Devin Gibbs

Frank L Conlon

Affiliations

Soon will be listed here.

Abstract

Background: The zinc-finger transcription factor CASZ1 is required for differentiation of a distinct population of cardiomyocytes during development. However, expression of Casz1 mRNA is detected throughout the developing heart, suggesting the spatial regulation of CASZ1 occurs at the protein level. Relatively little is known about posttranscriptional regulation of Casz1 in the heart.

Results: We generated antibodies that specifically recognize CASZ1 in developing Xenopus embryos, and performed immunofluorescence analysis of CASZ1 during cardiac development. CASZ1 was detected throughout the developing myocardium. CASZ1 was restricted to terminally differentiated cardiomyocytes, and was down-regulated in cells that re-enter the cell cycle. We determined that CASZ1 expression correlated with terminal differentiation in cardiac muscle cells, skeletal muscle cells, and lymph-heart musculature.

Conclusions: This study indicates that spatially distinct expression of CASZ1 protein may be due to posttranscriptional control of Casz1 mRNA during cardiac development. The results of this study provide insights into the role of Casz1 in cardiac function and in the differentiation of other cell types, including skeletal muscle and lymph heart.

Citing Articles

Role of the CASZ1 transcription factor in tissue development and disease.

Liu T, Li T, Ke S Eur J Med Res. 2023; 28(1):562.

PMID: 38053207 PMC: 10696751. DOI: 10.1186/s40001-023-01548-y.

: Current Implications in Cardiovascular Diseases and Cancers.

Jian H, Poetsch A Biomedicines. 2023; 11(7).

PMID: 37509718 PMC: 10377389. DOI: 10.3390/biomedicines11072079.

Identification of a Novel de Novo Variant in the CASZ1 Causing a Rare Type of Dilated Cardiomyopathy.

Orlova A, Guseva D, Ryzhkova O Int J Mol Sci. 2022; 23(20).

PMID: 36293425 PMC: 9603937. DOI: 10.3390/ijms232012506.

Targeting cardiomyocyte proliferation as a key approach of promoting heart repair after injury.

Li S, Ma W, Cai B Mol Biomed. 2022; 2(1):34.

PMID: 35006441 PMC: 8607366. DOI: 10.1186/s43556-021-00047-y.

CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MYOD and MYOG.

Liu Z, Zhang X, Lei H, Lam N, Carter S, Yockey O Nat Commun. 2020; 11(1):911.

PMID: 32060262 PMC: 7021771. DOI: 10.1038/s41467-020-14684-4.

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