Inhibitors of Differentiation-1 Promotes Nitrosopyrrolidine-induced Transformation of HPV 16-immortalized Cervical Epithelial Cell

Overview

Journal Cancer Sci

Specialty Oncology

Date 2014 Mar 18

PMID 24628854

Citations 4

Authors

Lingxia Xie

Jinke Li

Yi Zhang

Bao Liu

Xue Peng

Yong Lin

Wenming Xu

Lina Hu

Affiliations

Soon will be listed here.

Abstract

Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development. However, how Id-1 contributes to cervical carcinogenesis is unknown. In the present study, we used an in vitro transformation model to investigate the role of Id-1 in the transformation of cervical cells. Human papillomavirus (HPV)-immortalized cervical epithelial cells (H8) were successfully transformed by exposure to the carcinogen N-nitrosopyrrolidine (NPYR). The expression of both Id-1 RNA and protein was significantly increased in transformed H8 cells, suggesting a possible role of Id-1 in cervical cell transformation. Ectopic expression of Id-1 in H8 cells potentiated NPYR-induced cell transformation. In contrast, silencing of Id-1 suppressed NPYR-induced H8 cell transformation. In addition, the expression of HPV E6 and E7 oncoproteins was upregulated while that of the tumor suppressors p53 and pRb was suppressed after H8 cell transformation. Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

Citing Articles

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