The C-terminal Repeat Domains of NsP3 from the Old World Alphaviruses Bind Directly to G3BP

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2014 Mar 14

PMID 24623412

Citations 66

Authors

Marc D Panas

Tero Ahola

Gerald M McInerney

Affiliations

Soon will be listed here.

Abstract

The Old World alphaviruses block stress granule assembly by sequestration of RasGAP SH3-domain binding protein (G3BP). Here, we show that the proline-rich sequences in the hypervariable domain of nonstructural protein 3 (nsP3) of both Semliki Forest virus and Chikungunya virus were dispensable for binding to G3BP. nsP3 variants with or without this domain colocalized with G3BP. Furthermore, we show that the C-terminal repeat motifs of nsP3 were sufficient for G3BP binding.

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