Capped Poly(A) Leaders of Variable Lengths at the 5' Ends of Vaccinia Virus Late MRNAs

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Jan 1

PMID 2462059

Citations 32

Authors

B Y Ahn

B Moss

Affiliations

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Abstract

Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)nUpG. . ., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase T1, and RNase U2 digestion products.

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