A Novel Universal Multiplex PCR Improves Detection of AZFc Y-chromosome Microdeletions

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2014 Mar 12

PMID 24615019

Citations 6

Authors

Hong-Yun Zheng

Yan Li

Fu-Jin Shen

Yong-Qing Tong

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine the frequencies and the characteristics of Y chromosome microdeletions (pl) in infertile men from central China to perform appropriate therapeutic choices by updated multiplex-PCR.

Methods: In this study, we established a novel universal primer-multiplex-PCR (U-M-PCR) method to overcome the disadvantages of traditional multiplex PCR (M-PCR). We chose 15 sequence-tagged sites (STS) for detection of Y chromosome microdeletions. 540 infertile male patients and 100 healthy male controls were selected in the study.

Results: Of the 540 male infertility patients, 48 Y-chromosome microdeletions were detected, with a total deletion rate of 8.9 %. Of these deletions, the rate of AZFa deletions (sY84) was 0.5 % (3/540), the rate of AZFb deletions (sY143) was 0.7 % (4/540) and the rate of AZFc deletions (sY242, sY254 and sY255) was 7.6 % (41/540). Compared with AZF deletion rates by M-PCR, we found U-M-PCR could detect AZFc deletion more specifically (1.0 % & 7.6 %). No Y-chromosome microdeletions were detected in the 100 males with normal semen (the control group).

Conclusions: U-M-PCR method was more specific to detect AZFc microdeletions. It is necessary to use the U-M-PCR method to offer genetic screening and counseling to infertile men prior to intracytoplasmic sperm injection (ICSI) or in-vitro fertilization (IVF).

Citing Articles

Clinical Analysis of Y Chromosome Microdeletions and Chromosomal Aberrations in 1596 Male Infertility Patients of the Zhuang Ethnic Group in Guangxi.

Shi M, Ma S, Huang L, Huang C, Wang J, Qin X Reprod Sci. 2024; 31(10):3074-3085.

PMID: 38836967 PMC: 11438701. DOI: 10.1007/s43032-024-01568-x.

Optimization of Multiplex-PCR Technique To Determine Azf Deletions in infertility Male Patients.

Nguyen Thanh T, Tien S, Nguyen Van P, Thai S, Luong Cong T, Le T Int J Gen Med. 2024; 17:1579-1589.

PMID: 38690198 PMC: 11059631. DOI: 10.2147/IJGM.S455513.

External and Genetic Conditions Determining Male Infertility.

Kaminski P, Baszynski J, Jerzak I, Kavanagh B, Nowacka-Chiari E, Polanin M Int J Mol Sci. 2020; 21(15).

PMID: 32722328 PMC: 7432692. DOI: 10.3390/ijms21155274.

The Importance of Cytogenetics and Associated Molecular Techniques in the Management of Patients Carrying Robertsonian Translocation and Their Pregnancy Outcome by Intracytoplasmic Sperm Injection.

Kar B, Sivamani S, Kundavi S, Varma T J Obstet Gynaecol India. 2018; 68(2):93-97.

PMID: 29662277 PMC: 5895556. DOI: 10.1007/s13224-017-0999-y.

Azoospermia Factor C Subregion of the Y Chromosome.

Nailwal M, Chauhan J J Hum Reprod Sci. 2018; 10(4):256-260.

PMID: 29430151 PMC: 5799928. DOI: 10.4103/jhrs.JHRS_16_17.

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