Qualitative and Quantitative Contrast-enhanced Ultrasonographic Assessment of Cerulein-induced Acute Pancreatitis in Dogs

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2014 Mar 12

PMID 24612403

Citations 6

Authors

S Y Lim

K Nakamura

K Morishita

N Sasaki

M Murakami

T Osuga

H Ohta

M Yamasaki

M Takiguchi

Affiliations

Soon will be listed here.

Abstract

Background: Acute pancreatitis (AP) is the most common disease of the canine exocrine pancreas, and accurate noninvasive diagnosis is challenging.

Hypothesis/objectives: To determine the feasibility of using quantitative contrast-enhanced ultrasonography (CEUS) to detect pancreatic perfusional changes in cerulein-induced AP in dogs.

Animals: Six adult female Beagles.

Methods: Each dog received 2 hours of IV infusion with 7.5 μg/kg/h of cerulein diluted in saline. As control, all dogs received 2 hours of IV infusion of saline 2 weeks before cerulein infusion. CEUS of the pancreas and duodenum were performed before (0 hour), and at 2, 4, 6, and 12 hours after saline and cerulein infusion. Time-intensity curves were created from regions of interest in the pancreas and duodenum. Five perfusional parameters were measured for statistical analysis: time to initial up-slope, peak time, time to wash-out, peak intensity (PI), and area under the curve (AUC).

Results: In cerulein-induced AP, pancreatic PI increased at 2 and 4 hours when compared to 0 hour, and at 2, 4, and 6 hours when compared to control. AUC increased at 4 hours when compared to 0 hour, and at 2 and 4 hours when compared to control. Time to wash-out was prolonged at 4 hours when compared to control. For saline control, peak time was faster at 2 hours when compared to 0 hour.

Conclusions And Clinical Importance: CEUS parameters PI and AUC can provide useful information in differentiating acute pancreatitis from normal pancreas. Cerulein-induced AP was characterized by prolonged hyperechoic enhancement on CEUS.

Citing Articles

Candidate circulating microRNA biomarkers in dogs with chronic pancreatitis.

Armstrong S, Hunter R, Oosthyuzen W, Parys M, Gow A, Salavati Schmitz S J Vet Intern Med. 2024; 38(2):995-1004.

PMID: 38351718 PMC: 10937508. DOI: 10.1111/jvim.17009.

Comparison of pancreatic and renal blood flow in a canine tachycardia-induced cardiomyopathy model.

Yoshimura A, Ohmori T, Yamada S, Kawaguchi T, Kishimoto M, Iwanaga T J Vet Med Sci. 2020; 82(6):836-845.

PMID: 32336699 PMC: 7324827. DOI: 10.1292/jvms.19-0694.

Animal Models: Challenges and Opportunities to Determine Optimal Experimental Models of Pancreatitis and Pancreatic Cancer.

Saloman J, Albers K, Cruz-Monserrate Z, Davis B, Edderkaoui M, Eibl G Pancreas. 2019; 48(6):759-779.

PMID: 31206467 PMC: 6581211. DOI: 10.1097/MPA.0000000000001335.

Evaluation of Circulating MicroRNA Biomarkers in the Acute Pancreatic Injury Dog Model.

Lee H, Park H, Choi H, Lee S, Lee S, Lee J Int J Mol Sci. 2018; 19(10).

PMID: 30301227 PMC: 6212914. DOI: 10.3390/ijms19103048.

Contrast-enhanced ultrasonography of the pancreas in healthy cats.

Diana A, Linta N, Cipone M, Fedone V, Steiner J, Fracassi F BMC Vet Res. 2015; 11:64.

PMID: 25879918 PMC: 4367928. DOI: 10.1186/s12917-015-0380-2.

References

Okada M, Hoffmann C, Wolf K, Albrecht T . Bolus versus continuous infusion of microbubble contrast agent for liver US: initial experience. Radiology. 2005; 237(3):1063-7. DOI: 10.1148/radiol.2373041619. View

Xenoulis P, Steiner J . Canine and feline pancreatic lipase immunoreactivity. Vet Clin Pathol. 2012; 41(3):312-24. DOI: 10.1111/j.1939-165X.2012.00458.x. View

Ilkiw J . Other potentially useful new injectable anesthetic agents. Vet Clin North Am Small Anim Pract. 1992; 22(2):281-9. DOI: 10.1016/s0195-5616(92)50611-8. View

Golea A, Badea R, Socaciu M, Diaconu B, Iacob D . Quantitative analysis of tissue perfusion using contrast-enhanced transabdominal ultrasound (CEUS) in the evaluation of the severity of acute pancreatitis. Med Ultrason. 2011; 12(3):198-204. View

Su K, Cuthbertson C, Christophi C . Review of experimental animal models of acute pancreatitis. HPB (Oxford). 2008; 8(4):264-86. PMC: 2023897. DOI: 10.1080/13651820500467358. View

Kersting S, Konopke R, Kersting F, Volk A, Distler M, Bergert H . Quantitative perfusion analysis of transabdominal contrast-enhanced ultrasonography of pancreatic masses and carcinomas. Gastroenterology. 2009; 137(6):1903-11. DOI: 10.1053/j.gastro.2009.08.049. View

Renner I, Wisner Jr J . Ceruletide-induced acute pancreatitis in the dog and its amelioration by exogenous secretin. Int J Pancreatol. 1986; 1(1):39-49. DOI: 10.1007/BF02795238. View

Nakamura K, Takagi S, Sasaki N, Kumara W, Murakami M, Ohta H . Contrast-enhanced ultrasonography for characterization of canine focal liver lesions. Vet Radiol Ultrasound. 2010; 51(1):79-85. DOI: 10.1111/j.1740-8261.2009.01627.x. View

Moon M, Biller D, Armbrust L . Ultrasonographic appearance and etiology of corrugated small intestine. Vet Radiol Ultrasound. 2003; 44(2):199-203. DOI: 10.1111/j.1740-8261.2003.tb01271.x. View

10.

Morita Y, Takiguchi M, Yasuda J, Kitamura T, Syakalima M, Hashimoto A . Endoscopic and transcutaneous ultrasonographic findings and grey-scale histogram analysis in dogs with caerulein-induced pancreatitis. Vet Q. 1998; 20(3):89-92. DOI: 10.1080/01652176.1998.9694846. View

11.

Sontum P . Physicochemical characteristics of Sonazoid, a new contrast agent for ultrasound imaging. Ultrasound Med Biol. 2008; 34(5):824-33. DOI: 10.1016/j.ultrasmedbio.2007.11.006. View

12.

Watson P . Chronic pancreatitis in dogs. Top Companion Anim Med. 2012; 27(3):133-9. DOI: 10.1053/j.tcam.2012.04.006. View

13.

DOnofrio M, Zamboni G, Tognolini A, Malago R, Faccioli N, Frulloni L . Mass-forming pancreatitis: value of contrast-enhanced ultrasonography. World J Gastroenterol. 2006; 12(26):4181-4. PMC: 4087369. DOI: 10.3748/wjg.v12.i26.4181. View

14.

Lim S, Nakamura K, Morishita K, Sasaki N, Murakami M, Osuga T . Qualitative and quantitative contrast enhanced ultrasonography of the pancreas using bolus injection and continuous infusion methods in normal dogs. J Vet Med Sci. 2013; 75(12):1601-7. PMC: 3942965. DOI: 10.1292/jvms.13-0199. View

15.

Haers H, Daminet S, Smets P, Duchateau L, Aresu L, Saunders J . Use of quantitative contrast-enhanced ultrasonography to detect diffuse renal changes in Beagles with iatrogenic hypercortisolism. Am J Vet Res. 2012; 74(1):70-7. DOI: 10.2460/ajvr.74.1.70. View

16.

Waller K, OBrien R, Zagzebski J . Quantitative contrast ultrasound analysis of renal perfusion in normal dogs. Vet Radiol Ultrasound. 2007; 48(4):373-7. DOI: 10.1111/j.1740-8261.2007.00259.x. View

17.

Hess R, Saunders H, Van Winkle T, Shofer F, Washabau R . Clinical, clinicopathologic, radiographic, and ultrasonographic abnormalities in dogs with fatal acute pancreatitis: 70 cases (1986-1995). J Am Vet Med Assoc. 1998; 213(5):665-70. View

18.

DOnofrio M, Zamboni G, Faccioli N, Capelli P, Pozzi Mucelli R . Ultrasonography of the pancreas. 4. Contrast-enhanced imaging. Abdom Imaging. 2006; 32(2):171-81. DOI: 10.1007/s00261-006-9010-6. View

19.

Rickes S, Uhle C, Kahl S, Kolfenbach S, Monkemuller K, Effenberger O . Echo enhanced ultrasound: a new valid initial imaging approach for severe acute pancreatitis. Gut. 2005; 55(1):74-8. PMC: 1856359. DOI: 10.1136/gut.2005.070276. View

20.

McEntee G, Leahy A, Cottell D, Dervan P, McGEENEY K, Fitzpatrick J . Three-dimensional morphological study of the pancreatic microvasculature in caerulein-induced experimental pancreatitis. Br J Surg. 1989; 76(8):853-5. DOI: 10.1002/bjs.1800760830. View