The Effect of Omega Conotoxin GVIA, a Peptide Modulator of the N-type Voltage Sensitive Calcium Channels, on Motor Responses Produced by Activation of Efferent and Sensory Nerves in Mammalian Smooth Muscle

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1988 Aug 1

PMID 2460772

Citations 36

Authors

C A Maggi

R Patacchini

P Santicioli

I T Lippe

S Giuliani

P Geppetti

E Del Bianco

S Selleri

A Meli

Affiliations

Soon will be listed here.

Abstract

1. The effect of omega-conotoxin (CTX) GVIA, a peptide which blocks neuronal calcium channels, were investigated on nerve-mediated motor responses in a variety of isolated smooth muscle preparations from rats and guinea-pigs. 2. In the rat or guinea-pig isolated vas deferens CTX (1 nM-1 microM) produced a concentration and time-related inhibition of the response to field stimulation, while the responses to KCl, noradrenaline or adenosine triphosphate were unaffected. In the presence of CTX a series of tetrodotoxin-resistant contractions could be elicited by field stimulation by increasing pulse width and/or voltage. 3. In the rat or guinea-pig isolated urinary bladder, CTX produced a concentration and time-dependent inhibition of twitch responses to field stimulation without affecting the response to exogenous acetylcholine. In the rat bladder the maximal effect did not exceed 25% inhibition while a much larger fraction of the response (about 70%) was inhibited in the guinea-pig bladder. The CTX-resistant response was abolished, in both tissues, by tetrodotoxin. 4. The effects of CTX in the rat bladder were also studied with a whole range of frequencies of field stimulation (0.1-50 Hz). Maximal inhibition was observed toward contractions elicited at frequencies of 2-5 Hz. At low frequencies the inhibitory effects of CTX and atropine were almost additive while at high frequencies of stimulation a large component of the atropine-sensitive response was CTX-resistant. 5. In the rat isolated proximal duodenum, field stimulation in the presence of atropine and guanethidine produced a primary relaxation followed by a rebound contraction.(ABSTRACT TRUNCATED AT 250 WORDS)

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References

Miller R . Multiple calcium channels and neuronal function. Science. 1987; 235(4784):46-52. DOI: 10.1126/science.2432656. View

Maggi C, Santicioli P, Meli A . Postnatal development of micturition reflex in rats. Am J Physiol. 1986; 250(5 Pt 2):R926-31. DOI: 10.1152/ajpregu.1986.250.5.R926. View

Maggi C, Evangelista S, Grimaldi G, Santicioli P, Giolitti A, Meli A . Evidence for the involvement of arachidonic acid metabolites in spontaneous and drug-induced contractions of rat urinary bladder. J Pharmacol Exp Ther. 1984; 230(2):500-13. View

Sano K, Enomoto K, Maeno T . Effects of synthetic omega-conotoxin, a new type Ca2+ antagonist, on frog and mouse neuromuscular transmission. Eur J Pharmacol. 1987; 141(2):235-41. DOI: 10.1016/0014-2999(87)90268-8. View

Jancso G, Karcsu S, KIRALY E, Szebeni A, Toth L, Bacsy E . Neurotoxin induced nerve cell degeneration: possible involvement of calcium. Brain Res. 1984; 295(2):211-6. DOI: 10.1016/0006-8993(84)90969-7. View

Santicioli P, Patacchini R, Maggi C, Meli A . Exposure to calcium-free medium protects sensory fibers by capsaicin desensitization. Neurosci Lett. 1987; 80(2):167-72. DOI: 10.1016/0304-3940(87)90648-3. View

Maggi C, Giuliani S, Manzini S, Santicioli P, Meli A . Motor effect of neurokinins on the rat duodenum: evidence for the involvement of substance K and substance P receptors. J Pharmacol Exp Ther. 1986; 238(1):341-51. View

Olivera B, McIntosh J, Cruz L, Luque F, Gray W . Purification and sequence of a presynaptic peptide toxin from Conus geographus venom. Biochemistry. 1984; 23(22):5087-90. DOI: 10.1021/bi00317a001. View

Olivera B, Gray W, Zeikus R, McIntosh J, Varga J, Rivier J . Peptide neurotoxins from fish-hunting cone snails. Science. 1985; 230(4732):1338-43. DOI: 10.1126/science.4071055. View

10.

Marsh S, Stansfeld C, Brown D, Davey R, McCarthy D . The mechanism of action of capsaicin on sensory C-type neurons and their axons in vitro. Neuroscience. 1987; 23(1):275-89. DOI: 10.1016/0306-4522(87)90289-2. View

11.

Maggi C, Patacchini R, Santicioli P, Giuliani S, Geppetti P, Meli A . Protective action of ruthenium red toward capsaicin desensitization of sensory fibers. Neurosci Lett. 1988; 88(2):201-5. DOI: 10.1016/0304-3940(88)90126-7. View

12.

Gamse R, Molnar A, Lembeck F . Substance P release from spinal cord slices by capsaicin. Life Sci. 1979; 25(7):629-36. DOI: 10.1016/0024-3205(79)90558-7. View

13.

Maggi C, Santicioli P, Meli A . Pharmacological evidence for the existence of two components in the twitch response to field stimulation of detrusor strips from the rat urinary bladder. J Auton Pharmacol. 1985; 5(3):221-9. DOI: 10.1111/j.1474-8673.1985.tb00123.x. View

14.

Maggi C, Giuliani S, Santicioli P, Abelli L, Geppetti P, Somma V . Species-related variations in the effects of capsaicin on urinary bladder functions: relation to bladder content of substance P-like immunoreactivity. Naunyn Schmiedebergs Arch Pharmacol. 1987; 336(5):546-55. DOI: 10.1007/BF00169312. View

15.

Maggi C, Manzini S, Giuliani S, Santicioli P, Meli A . Extrinsic origin of the capsaicin-sensitive innervation of rat duodenum: possible involvement of calcitonin gene-related peptide (CGRP) in the capsaicin-induced activation of intramural non-adrenergic non-cholinergic neurons. Naunyn Schmiedebergs Arch Pharmacol. 1986; 334(2):172-80. DOI: 10.1007/BF00505818. View

16.

Tapia R, Meza-Ruiz G . Inhibition by ruthenium red of the calcium-dependent release of [3H]GABA in synaptosomal fractions. Brain Res. 1977; 126(1):160-6. DOI: 10.1016/0006-8993(77)90223-2. View

17.

Maggi C, Santicioli P, Geppetti P, Furio M, Frilli S, Conte B . The contribution of capsaicin-sensitive innervation to activation of the spinal vesico-vesical reflex in rats: relationship between substance P levels in the urinary bladder and the sensory-efferent function of capsaicin-sensitive sensory neurons. Brain Res. 1987; 415(1):1-13. DOI: 10.1016/0006-8993(87)90263-0. View

18.

Maggi C, Santicioli P, Geppetti P, Giuliani S, Patacchini R, Frilli S . Involvement of a peripheral site of action in the early phase of neuropeptide depletion following capsaicin desensitization. Brain Res. 1987; 436(2):402-6. DOI: 10.1016/0006-8993(87)91688-x. View

19.

Augustine G, Charlton M, Smith S . Calcium action in synaptic transmitter release. Annu Rev Neurosci. 1987; 10:633-93. DOI: 10.1146/annurev.ne.10.030187.003221. View

20.

Kerr L, Yoshikami D . A venom peptide with a novel presynaptic blocking action. Nature. 1984; 308(5956):282-4. DOI: 10.1038/308282a0. View