Fluoxetine Induces Proliferation and Inhibits Differentiation of Hypothalamic Neuroprogenitor Cells in Vitro

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Mar 7

PMID 24598761

Citations 7

Authors

Ligia Sousa-Ferreira

Celia Aveleira

Mariana Botelho

Ana Rita Alvaro

Luis Pereira de Almeida

Claudia Cavadas

Affiliations

Soon will be listed here.

Abstract

A significant number of children undergo maternal exposure to antidepressants and they often present low birth weight. Therefore, it is important to understand how selective serotonin reuptake inhibitors (SSRIs) affect the development of the hypothalamus, the key center for metabolism regulation. In this study we investigated the proliferative actions of fluoxetine in fetal hypothalamic neuroprogenitor cells and demonstrate that fluoxetine induces the proliferation of these cells, as shown by increased neurospheres size and number of proliferative cells (Ki-67+ cells). Moreover, fluoxetine inhibits the differentiation of hypothalamic neuroprogenitor cells, as demonstrated by decreased number of mature neurons (Neu-N+ cells) and increased number of undifferentiated cells (SOX-2+ cells). Additionally, fluoxetine-induced proliferation and maintenance of hypothalamic neuroprogenitor cells leads to changes in the mRNA levels of appetite regulator neuropeptides, including Neuropeptide Y (NPY) and Cocaine-and-Amphetamine-Regulated-Transcript (CART). This study provides the first evidence that SSRIs affect the development of hypothalamic neuroprogenitor cells in vitro with consequent alterations on appetite neuropeptides.

Citing Articles

Olanzapine add-on treatment promotes neuronal differentiation of neural stem cells compared with fluoxetine alone.

Sun J, Wu D, Xu G, Chen F, Ding X, Xie L Biosci Rep. 2022; 42(10).

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Change of hypothalamic adult neurogenesis in mice by chronic treatment of fluoxetine.

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Depression and Antidepressants During Pregnancy: Craniofacial Defects Due to Stem/Progenitor Cell Deregulation Mediated by Serotonin.

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PMID: 34476233 PMC: 8406697. DOI: 10.3389/fcell.2021.632766.

Fluoxetine Inhibits Canonical Wnt Signaling to Impair Embryoid Body Morphogenesis: Potential Teratogenic Mechanisms of a Commonly Used Antidepressant.

Warkus E, Marikawa Y Toxicol Sci. 2018; 165(2):372-388.

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Harmine stimulates proliferation of human neural progenitors.

Dakic V, de Moraes Maciel R, Drummond H, M Nascimento J, Trindade P, K Rehen S PeerJ. 2016; 4:e2727.

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