Unusual Calcium-activated Potassium Channels of Bovine Parathyroid Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Oct 1

PMID 2459700

Citations 7

Authors

M Jia

G Ehrenstein

K Iwasa

Affiliations

Soon will be listed here.

Abstract

Parathyroid cells have unusual responses to an increase in the external calcium concentration--a reduction in secretion and a depolarization. In an attempt to explain this behavior, we have studied voltage-clamped inside-out patches from bovine parathyroid cells. We found a potassium-selective channel that requires internal calcium to open and has a 175-pS conductance. This channel differs from calcium-activated potassium channels that have been found in other cells in that it closes when the internal calcium concentration is increased above about 160 nM. This channel can account for the depolarization that occurs in parathyroid cells in high-calcium solutions. It may also account for the reduced secretion in high-calcium solutions.

Citing Articles

High extracellular Ca2+ stimulates Ca2+-activated Cl- currents in frog parathyroid cells through the mediation of arachidonic acid cascade.

Okada Y, Imendra K, Miyazaki T, Hotokezaka H, Fujiyama R, Toda K PLoS One. 2011; 6(4):e19158.

PMID: 21559478 PMC: 3084778. DOI: 10.1371/journal.pone.0019158.

Molecular basis of fast inactivation in voltage and Ca2+-activated K+ channels: a transmembrane beta-subunit homolog.

Wallner M, Meera P, Toro L Proc Natl Acad Sci U S A. 1999; 96(7):4137-42.

PMID: 10097176 PMC: 22433. DOI: 10.1073/pnas.96.7.4137.

Activity-dependent modulation of the presynaptic potassium current in the frog neuromuscular junction.

Miralles F, Solsona C J Physiol. 1996; 495 ( Pt 3):717-32.

PMID: 8887778 PMC: 1160777. DOI: 10.1113/jphysiol.1996.sp021628.

X-ray microanalysis of elemental changes in human parathyroid glands in primary and secondary hyperparathyroidism.

WROBLEWSKI R, Johansson H, Grimelius L Histochem Cell Biol. 1996; 105(6):467-73.

PMID: 8791106 DOI: 10.1007/BF01457660.

Intracellular Ca2+ inactivates an outwardly rectifying K+ current in human adenomatous parathyroid cells.

Komwatana P, Conigrave A, Delbridge L, Young J, Cook D Pflugers Arch. 1994; 426(3-4):320-7.

PMID: 8183643 DOI: 10.1007/BF00374788.

References

Sherwood L, MAYER G, Ramberg Jr C, Kronfeld D, Aurbach G, Potts Jr J . Regulation of parathyroid hormone secretion: proportional control by calcium, lack of effect of phosphate. Endocrinology. 1968; 83(5):1043-51. DOI: 10.1210/endo-83-5-1043. View

Meech R . The sensitivity of Helix aspersa neurones to injected calcium ions. J Physiol. 1974; 237(2):259-77. PMC: 1350883. DOI: 10.1113/jphysiol.1974.sp010481. View

Bruce B, ANDERSON Jr N . Hyperpolarization in mouse parathyroid cells by low calcium. Am J Physiol. 1979; 236(1):C15-21. DOI: 10.1152/ajpcell.1979.236.1.C15. View

Fabiato A, Fabiato F . Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris). 1979; 75(5):463-505. View

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Wong B, Lecar H, Adler M . Single calcium-dependent potassium channels in clonal anterior pituitary cells. Biophys J. 1982; 39(3):313-7. PMC: 1328949. DOI: 10.1016/S0006-3495(82)84522-0. View

Rahamimoff R, DeRiemer S, Sakmann B, Stadler H, Yakir N . Ion channels in synaptic vesicles from Torpedo electric organ. Proc Natl Acad Sci U S A. 1988; 85(14):5310-4. PMC: 281740. DOI: 10.1073/pnas.85.14.5310. View

Maruyama Y, Petersen O, Flanagan P, Pearson G . Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells. Nature. 1983; 305(5931):228-32. DOI: 10.1038/305228a0. View

Lopez-Barneo J, ARMSTRONG C . Depolarizing response of rat parathyroid cells to divalent cations. J Gen Physiol. 1983; 82(2):269-94. PMC: 2228694. DOI: 10.1085/jgp.82.2.269. View

10.

Vergara C, Latorre R . Kinetics of Ca2+-activated K+ channels from rabbit muscle incorporated into planar bilayers. Evidence for a Ca2+ and Ba2+ blockade. J Gen Physiol. 1983; 82(4):543-68. PMC: 2228658. DOI: 10.1085/jgp.82.4.543. View

11.

Shoback D, Thatcher J, Leombruno R, Brown E . Relationship between parathyroid hormone secretion and cytosolic calcium concentration in dispersed bovine parathyroid cells. Proc Natl Acad Sci U S A. 1984; 81(10):3113-7. PMC: 345231. DOI: 10.1073/pnas.81.10.3113. View

12.

Iwatsuki N, Petersen O . Inhibition of Ca2+-activated K+ channels in pig pancreatic acinar cells by Ba2+, Ca2+, quinine and quinidine. Biochim Biophys Acta. 1985; 819(2):249-57. DOI: 10.1016/0005-2736(85)90180-4. View

13.

Fitzpatrick L, Brandi M, Aurbach G . Prostaglandin F2 alpha and alpha-adrenergic agonists regulate parathyroid cell function via the inhibitory guanine nucleotide regulatory protein. Endocrinology. 1986; 118(5):2115-9. DOI: 10.1210/endo-118-5-2115. View

14.

Brown E . PTH secretion in vivo and in vitro. Regulation by calcium and other secretagogues. Miner Electrolyte Metab. 1982; 8(3-4):130-50. View