Evidence for Apical Chloride Channels in Rabbit Mandibular Salivary Glands. A Chloride-selective Microelectrode Study

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1988 Jun 1

PMID 2457871

Citations 4

Authors

K R Lau

R M Case

Affiliations

Soon will be listed here.

Abstract

Double-barrelled, chloride-selective microelectrodes were used to study mandibular gland acinar cells at rest and during cholinergic stimulation. At rest, intracellular chloride activity was five times the expected equilibrium activity. During sustained stimulation with acetylcholine, chloride activity fell to three times the expected equilibrium activity. Thus, the gradient for chloride exit was reduced in the stimulated cell. These results lead to the conclusion that stimulation increases the permeability of the acinar cell to chloride. Experiments in which extracellular chloride was removed provided evidence that the permeability increase was due to opening of chloride channels located principally in the apical membrane of the acinar cell.

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References

Case R, Conigrave A, Novak I, Young J . Electrolyte and protein secretion by the perfused rabbit mandibular gland stimulated with acetylcholine or catecholamines. J Physiol. 1980; 300:467-87. PMC: 1279366. DOI: 10.1113/jphysiol.1980.sp013173. View

Findlay I, Petersen O . Acetylcholine stimulates a Ca2+-dependent C1- conductance in mouse lacrimal acinar cells. Pflugers Arch. 1985; 403(3):328-30. DOI: 10.1007/BF00583609. View

Christoffersen C, Skibsted L . Calcium ion activity in physiological salt solutions: influence of anions substituted for chloride. Comp Biochem Physiol A Comp Physiol. 1975; 52(2):317-22. DOI: 10.1016/s0300-9629(75)80094-6. View

Welsh M, Smith P, Frizzell R . Chloride secretion by canine tracheal epithelium: III. Membrane resistances and electromotive forces. J Membr Biol. 1983; 71(3):209-18. DOI: 10.1007/BF01875462. View

Murakami M, Novak I, Young J . Choline evokes fluid secretion by perfused rat mandibular gland without desensitization. Am J Physiol. 1986; 251(1 Pt 1):G84-9. DOI: 10.1152/ajpgi.1986.251.1.G84. View

Bijman J, Cook D, Van Os C . Effect of amiloride on electrolyte transport parameters of the main duct of the rabbit mandibular salivary gland. Pflugers Arch. 1983; 398(2):96-102. DOI: 10.1007/BF00581055. View

Young J, Martin C . The effect of a sympatho- and a parasympathomimetic drug on the electrolyte concentrations of primary and final saliva of the rat submaxillary gland. Pflugers Arch. 1971; 327(4):285-302. DOI: 10.1007/BF00588449. View

Martinez J, Cassity N . Effects of 4,4'-diisothiocyano-2,2'-stilbene disulphonic acid and amiloride on salivary secretion by isolated, perfused rat submandibular glands. Arch Oral Biol. 1985; 30(11-12):797-803. DOI: 10.1016/0003-9969(85)90134-7. View

Fromm M, Schultz S . Some properties of KCl-filled microelectrodes: correlation of potassium "leakage" with tip resistance. J Membr Biol. 1981; 62(3):239-44. DOI: 10.1007/BF01998169. View

10.

Novak I, Young J . Two independent anion transport systems in rabbit mandibular salivary glands. Pflugers Arch. 1986; 407(6):649-56. DOI: 10.1007/BF00582647. View

11.

Case R, Hunter M, Novak I, Young J . The anionic basis of fluid secretion by the rabbit mandibular salivary gland. J Physiol. 1984; 349:619-30. PMC: 1199358. DOI: 10.1113/jphysiol.1984.sp015177. View

12.

Butcher F, Putney Jr J . Regulation of parotid gland function by cyclic nucleotides and calcium. Adv Cyclic Nucleotide Res. 1980; 13:215-49. View

13.

Hunter M, Smith P, Case R . The dependence of fluid secretion by mandibular salivary gland and pancreas on extracellular calcium. Cell Calcium. 1983; 4(4):307-17. DOI: 10.1016/0143-4160(83)90007-6. View

14.

Saito Y, Ozawa T, Hayashi H, Nishiyama A . The effect of acetylcholine on chloride transport across the mouse lacrimal gland acinar cell membranes. Pflugers Arch. 1987; 409(3):280-8. DOI: 10.1007/BF00583477. View

15.

Greger R, Schlatter E, Wang F, Forrest Jr J . Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). III. Effects of stimulation of secretion by cyclic AMP. Pflugers Arch. 1984; 402(4):376-84. DOI: 10.1007/BF00583938. View

16.

Nishiyama A, Petersen O . Membrane potential and resistance measurement in acinar cells from salivary glands in vitro: effect of acetylcholine. J Physiol. 1974; 242(1):173-88. PMC: 1330606. DOI: 10.1113/jphysiol.1974.sp010700. View

17.

Reuss L, Reinach P, Weinman S, Grady T . Intracellular ion activities and Cl-transport mechanisms in bullfrog corneal epithelium. Am J Physiol. 1983; 244(5):C336-47. DOI: 10.1152/ajpcell.1983.244.5.C336. View

18.

Marty A, Tan Y, Trautmann A . Three types of calcium-dependent channel in rat lacrimal glands. J Physiol. 1984; 357:293-325. PMC: 1193259. DOI: 10.1113/jphysiol.1984.sp015501. View

19.

Petersen O . Calcium-activated potassium channels and fluid secretion by exocrine glands. Am J Physiol. 1986; 251(1 Pt 1):G1-13. DOI: 10.1152/ajpgi.1986.251.1.G1. View

20.

THAYSEN J, THORN N, SCHWARTZ I . Excretion of sodium, potassium, chloride and carbon dioxide in human parotid saliva. Am J Physiol. 1954; 178(1):155-9. DOI: 10.1152/ajplegacy.1954.178.1.155. View