Dramatic Reduction of Culture Time of Mycobacterium Tuberculosis

Overview

Journal Sci Rep

Specialty Science

Date 2014 Mar 1

PMID 24577292

Citations 35

Authors

Ramzi Ghodbane

Didier Raoult

Michel Drancourt

Affiliations

Soon will be listed here.

Abstract

Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture.

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