Dimethyl Sulfoxide Decreases the Fluorescence Yield of the Reaction Between Histamine and Ortho-phthalaldehyde and May Influence Histamine Release

Overview

Journal Agents Actions

Specialty Pharmacology

Date 1988 Jun 1

PMID 2457301

Citations 2

Authors

P Moldt

W K Andersen

S B Christensen

Affiliations

Soon will be listed here.

Abstract

A sensitive method for quantifying histamine is the assay based on the fluorescence of the product obtained after reacting the amine with ortho-phthalaldehyde. The presence of dimethyl sulfoxide (DMSO) even in concentrations as low as 1% decreases the yield of fluorescence. Neglecting this quenching leads to the erroneous conclusion that DMSO in a concentration dependent manner inhibits the thapsigargin induced histamine release from peritoneal rat mast cells. Apart from the release induced by the selective calcium ion mobilizers thapsigargin and ionophore A23147, DMSO potently inhibits the response to other secretagogues.

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