A Combination of Wnt and Growth Factor Signaling Induces Arl4c Expression to Form Epithelial Tubular Structures

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2014 Feb 25

PMID 24562386

Citations 41

Authors

Shinji Matsumoto

Shinsuke Fujii

Akira Sato

Souji Ibuka

Yoshinori Kagawa

Masaru Ishii

Akira Kikuchi

Affiliations

Soon will be listed here.

Abstract

Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.

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