Laminar and Temporal Expression Dynamics of Coding and Noncoding RNAs in the Mouse Neocortex

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2014 Feb 25

PMID 24561256

Citations 47

Authors

Sofia Fertuzinhos

Mingfeng Li

Yuka Imamura Kawasawa

Vedrana Ivic

Daniel Franjic

Darshani Singh

Michael Crair

Nenad Sestan

Affiliations

Soon will be listed here.

Abstract

The hallmark of the cerebral neocortex is its organization into six layers, each containing a characteristic set of cell types and synaptic connections. The transcriptional events involved in laminar development and function still remain elusive. Here, we employed deep sequencing of mRNA and small RNA species to gain insights into transcriptional differences among layers and their temporal dynamics during postnatal development of the mouse primary somatosensory neocortex. We identify a number of coding and noncoding transcripts with specific spatiotemporal expression and splicing patterns. We also identify signature trajectories and gene coexpression networks associated with distinct biological processes and transcriptional overlap between these processes. Finally, we provide data that allow the study of potential miRNA and mRNA interactions. Overall, this study provides an integrated view of the laminar and temporal expression dynamics of coding and noncoding transcripts in the mouse neocortex and a resource for studies of neurodevelopment and transcriptome.

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