RNA-DNA Differences Are Generated in Human Cells Within Seconds After RNA Exits Polymerase II

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2014 Feb 25

PMID 24561252

Citations 41

Authors

Isabel X Wang

Leighton J Core

Hojoong Kwak

Lauren Brady

Alan Bruzel

Lee McDaniel

Allison L Richards

Ming Wu

Christopher Grunseich

John T Lis

Vivian G Cheung

Affiliations

Soon will be listed here.

Abstract

RNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains ~55 nt from the RNA polymerase II (Pol II) active site. These RDDs occur so soon after transcription that they are incompatible with known deaminase-mediated RNA-editing mechanisms. Moreover, the 55 nt delay in appearance indicates that they do not arise during RNA synthesis by Pol II or as a direct consequence of modified base incorporation. Preliminary data suggest that RDD and R-loop formations may be coupled. These findings identify sequence substitution as an early step in cotranscriptional RNA processing.

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