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The Bioassay of Gibberellins

Overview
Journal Planta
Specialty Biology
Date 2014 Feb 21
PMID 24554208
Citations 47
Authors
R L Jones
J E Varner
Affiliations
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Abstract

A bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: 1) Release of α-amylase is one step closer to the primary site of action of GA; 2) release of α-amylase is not affected by solvent residues and is apparently completely specific for gibberellin; 3) release of α-amylase is not affected by substances other than gibberellins present in crude plant extracts.

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References
1.
Varner J, Chandra G . HORMONAL CONTROL OF ENZYME SYNTHESIS IN BARLEY ENDOSPERM. Proc Natl Acad Sci U S A. 1964; 52(1):100-6. PMC: 300580. DOI: 10.1073/pnas.52.1.100. View
2.
Paleg L . Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley Endosperm. Plant Physiol. 1960; 35(3):293-9. PMC: 405961. DOI: 10.1104/pp.35.3.293. View
3.
Paleg L, Aspinall D, Coombe B, Nicholls P . Physiological Effects of Gibberellic Acid. VI. Other Gibberellins in Three Test Systems. Plant Physiol. 1964; 39(2):286-90. PMC: 550070. DOI: 10.1104/pp.39.2.286. View
4.
Wilson C . Bacteria, antibiotics and amino Acid incorporation into maize endosperm protein bodies. Plant Physiol. 1966; 41(2):325-7. PMC: 1086340. DOI: 10.1104/pp.41.2.325. View