Assessment of Bax and Bcl-2 Immunoexpression in Patients with Oral Lichen Planus and Oral Squamous Cell Carcinoma

Overview

Journal Int J Mol Cell Med

Specialty Genetics

Date 2014 Feb 20

PMID 24551804

Citations 6

Authors

Shima Nafarzadeh

Sina Jafari

Ali Bijani

Affiliations

Soon will be listed here.

Abstract

Lichen planus (LP) is a chronic inflammatory disease of probable immune-based etiology. The pathogenesis of LP is unclear, but apoptotic changes in epidermal (epithelial) cells have been reported. Destruction of the basal cell layer is observed and many changes in cell proliferation, cell repair and cell death occur in the injured mucosal epithelium. The aim of this study was to evaluate and compare the expression of bax and bcl-2 in oral lichen planus (OLP), well differentiated oral squamous cell carcinoma (WOSCC) and normal mucosa. Sixty one paraffin-embedded biopsy including 11 cases of WOSCC, 30 cases of OLP (n=15 erosive OLP [OLP-E], n=15 reticular OLP [OLP-R]) and 20 normal mucosa were entered in our research. We used immunohistochemistry staining method for assessing bax and bcl-2 expression in epithelial layers. The percentage of stained cells was estimated in 5 randomized microscopic fields and classified as (-): 0%, (+) :< 10%, (++): 10-25%, (+++): 26-50%, (++++): > 50% positive cells. The data were analyzed with Mann-Whitney, Chi Square, and Kruskal-Wallis tests. Significant differences in bax expression were observed among OLP, WOSCC compared to normal mucosa (P=0.008). No significant difference in bax expression between OLP-E and OLP-R compared to WOSCC was seen (P>0.05). Bcl-2 was negative for all OLP and normal mucosa samples, and weak positivity was observed in WOSCC samples. According to the findings of our study, it may be possible to correlate the difference of bax and bcl-2 expression levels among the mentioned lesions to the malignant potential of OLP.

Citing Articles

A Molecular Hypothesis on Malignant Transformation of Oral Lichen Planus: A Systematic Review and Meta-Analysis of Cancer Hallmarks Expression in This Oral Potentially Malignant Disorder.

Keim-Del Pino C, Ramos-Garcia P, Gonzalez-Moles M Cancers (Basel). 2024; 16(15).

PMID: 39123342 PMC: 11311016. DOI: 10.3390/cancers16152614.

Hallmarks of Cancer Expression in Oral Lichen Planus: A Scoping Review of Systematic Reviews and Meta-Analyses.

Gonzalez-Moles M, Keim-Del Pino C, Ramos-Garcia P Int J Mol Sci. 2022; 23(21).

PMID: 36361889 PMC: 9658487. DOI: 10.3390/ijms232113099.

Significance of immunohistochemistry biomarkers in prediction of malignant transformation of oral lichen planus: A systematic review.

Al-Jamaei A, Subramanyam R, Helder M, Forouzanfar T, Ruslin M, Meij E Med Oral Patol Oral Cir Bucal. 2022; 27(5):e480-e488.

PMID: 35975803 PMC: 9445608. DOI: 10.4317/medoral.25491.

Probiotics as Potential Biological Immunomodulators in the Management of Oral Lichen Planus: What's New?.

Zanetta P, Ormelli M, Amoruso A, Pane M, Azzimonti B, Squarzanti D Int J Mol Sci. 2022; 23(7).

PMID: 35408849 PMC: 8998608. DOI: 10.3390/ijms23073489.

Down-regulation of miRNA-27b-3p suppresses keratinocytes apoptosis in oral lichen planus.

Chen J, Wang Y, Du G, Zhang W, Cao T, Shi L J Cell Mol Med. 2019; 23(6):4326-4337.

PMID: 30973209 PMC: 6533518. DOI: 10.1111/jcmm.14324.

References

Lage D, Pimentel V, Soares T, Souza E, Metze K, Cintra M . Perforin and granzyme B expression in oral and cutaneous lichen planus - a comparative study. J Cutan Pathol. 2011; 38(12):973-8. DOI: 10.1111/j.1600-0560.2011.01781.x. View

Teni T, Pawar S, Sanghvi V, Saranath D . Expression of bcl-2 and bax in chewing tobacco-induced oral cancers and oral lesions from India. Pathol Oncol Res. 2002; 8(2):109-14. DOI: 10.1007/BF03033719. View

Loro L, Johannessen A, Vintermyr O . Decreased expression of bcl-2 in moderate and severe oral epithelia dysplasias. Oral Oncol. 2002; 38(7):691-8. DOI: 10.1016/s1368-8375(02)00002-7. View

Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S, Sameshima M . The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 1991; 66(2):233-43. DOI: 10.1016/0092-8674(91)90614-5. View

Eisenberg E, Krutchkoff D . Lichenoid lesions of oral mucosa. Diagnostic criteria and their importance in the alleged relationship to oral cancer. Oral Surg Oral Med Oral Pathol. 1992; 73(6):699-704. DOI: 10.1016/0030-4220(92)90013-g. View

Tanda N, Mori S, Saito K, Ikawa K, Sakamoto S . Expression of apoptotic signaling proteins in leukoplakia and oral lichen planus: quantitative and topographical studies. J Oral Pathol Med. 2000; 29(8):385-93. DOI: 10.1034/j.1600-0714.2000.290804.x. View

Lee J, Kuo M, Cheng S, Chiang C, Jeng J, Chang H . Higher expressions of p53 and proliferating cell nuclear antigen (PCNA) in atrophic oral lichen planus and patients with areca quid chewing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 99(4):471-8. DOI: 10.1016/j.tripleo.2004.10.018. View

Lodi G, Scully C, Carrozzo M, Griffiths M, Sugerman P, Thongprasom K . Current controversies in oral lichen planus: report of an international consensus meeting. Part 2. Clinical management and malignant transformation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 100(2):164-78. DOI: 10.1016/j.tripleo.2004.06.076. View

de Sousa F, Paradella T, Carvalho Y, Rosa L . Comparative analysis of the expression of proliferating cell nuclear antigen, p53, bax, and bcl-2 in oral lichen planus and oral squamous cell carcinoma. Ann Diagn Pathol. 2009; 13(5):308-12. DOI: 10.1016/j.anndiagpath.2009.06.001. View

10.

Bascones-Ilundain C, Gonzalez-Moles M, Campo-Trapero J, Gil-Montoya J, Esparza-Gomez G, Cano-Sanchez J . No differences in caspase-3 and Bax expression in atrophic-erosive vs. reticular oral lichen planus. J Eur Acad Dermatol Venereol. 2008; 22(2):204-12. DOI: 10.1111/j.1468-3083.2007.02387.x. View

11.

Krutchkoff D, Cutler L, Laskowski S . Oral lichen planus: the evidence regarding potential malignant transformation. J Oral Pathol. 1978; 7(1):1-7. DOI: 10.1111/j.1600-0714.1978.tb01879.x. View

12.

Chen X, Wu Y, Li J, Liu Z, Yue Q, Liu H . Expression of apoptosis related protein in skin lesions of lichen planus and its implication. J Huazhong Univ Sci Technolog Med Sci. 2008; 28(3):349-51. DOI: 10.1007/s11596-008-0328-x. View

13.

van der Meij E, Schepman K, van der Waal I . The possible premalignant character of oral lichen planus and oral lichenoid lesions: a prospective study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96(2):164-71. DOI: 10.1016/s1079-2104(03)00305-6. View

14.

van der Meij E, Mast H, van der Waal I . The possible premalignant character of oral lichen planus and oral lichenoid lesions: a prospective five-year follow-up study of 192 patients. Oral Oncol. 2006; 43(8):742-8. DOI: 10.1016/j.oraloncology.2006.09.006. View

15.

Kim S, Chae C, Cho B, Kim H, Kim H, Kim I . Apoptosis of oral epithelial cells in oral lichen planus caused by upregulation of BMP-4. J Oral Pathol Med. 2006; 35(1):37-45. DOI: 10.1111/j.1600-0714.2005.00373.x. View

16.

Axell T, PINDBORG J, Smith C, van der Waal I . Oral white lesions with special reference to precancerous and tobacco- related lesions: conclusions of an international symposium held in Uppsala, Sweden, May 18-21 1994. International Collaborative Group on Oral White Lesions. J Oral Pathol Med. 1996; 25(2):49-54. DOI: 10.1111/j.1600-0714.1996.tb00191.x. View

17.

Eisen D . The clinical features, malignant potential, and systemic associations of oral lichen planus: a study of 723 patients. J Am Acad Dermatol. 2002; 46(2):207-14. DOI: 10.1067/mjd.2002.120452. View

18.

Abdel-Latif A, Abuel-Ela H, El-Shourbagy S . Increased caspase-3 and altered expression of apoptosis-associated proteins, Bcl-2 and Bax in lichen planus. Clin Exp Dermatol. 2009; 34(3):390-5. DOI: 10.1111/j.1365-2230.2008.03029.x. View

19.

Jordan R, Catzavelos G, Barrett A, Speight P . Differential expression of bcl-2 and bax in squamous cell carcinomas of the oral cavity. Eur J Cancer B Oral Oncol. 1996; 32B(6):394-400. DOI: 10.1016/s0964-1955(96)00033-4. View

20.

Kramer I, LUCAS R, PINDBORG J, Sobin L . Definition of leukoplakia and related lesions: an aid to studies on oral precancer. Oral Surg Oral Med Oral Pathol. 1978; 46(4):518-39. View