Downregulation of MMP2 and Bcl-2 in Adipose Derived Stem Cells (ASCs) Following Transfection with IP-10 Gene

Overview

Journal Avicenna J Med Biotechnol

Specialty Biomedical Engineering

Date 2014 Feb 20

PMID 24551432

Citations 3

Authors

Mahboobeh Razmkhah

Mansooreh Jaberipour

Abbas Ghaderi

Affiliations

Soon will be listed here.

Abstract

Background: Mesenchymal Stem Cells (MSCs) are recently introduced as novel immunological gene carriers for treatment of cancer. It is believed that balance between the expression of angiogenic and anti-angiogenic factors, such as SDF-1 and IP-10, may regulate neovascularization within the tumor.

Methods: In this study, we compared the expression of important tumor promoting mediators in IP-10-transfected Adipose Derived Stem Cells (ASCs) to those transfected with SDF-1. ASCs were isolated from adipose tissue of a normal subject undergoing cosmetic mamoplasty surgery using collagenase. ASCs were transfected with IP-10 or SDF-1 propagated plasmids by electroporation method and Lipofectamin 2000. Expressions of SDF-1, CXCR4, IP-10, Bcl-2, MMP2, IL-10, IGF-1, and VEGF were detected in transfected ASCs using quantitative Real-Time Polymerase Chain Reaction (qRT-PCR).

Results: Results showed that the expressions of SDF-1, CXCR4, Bcl-2, MMP2, IL-10, IGF-1, and VEGF were upregulated in SDF-1-transfected ASCs. In contrast, Bcl-2 and MMP2 transcripts showed 45×10(3) and 10 fold lower expression in ASCs transfected with IP-10 compared to non-transfected cells.

Conclusion: Anti-angiogenic chemokines such as IP-10 may modulate tumor promoting properties of ASCs and would be introduced as novel candidates for tumor immunotherapy; however, further studies are needed to be conducted.

Citing Articles

IP10-CDR3 Reduces The Viability And Induces The Apoptosis Of Ovarian Cancer Cells By Down-Regulating The Expression Of Bcl-2 And Caspase 3.

Chen Q, He Q, Zhuang L, Wang K, Yin C, He L Onco Targets Ther. 2020; 12:9697-9706.

PMID: 32009802 PMC: 6859960. DOI: 10.2147/OTT.S209757.

Engineered Mesenchymal Stem Cells as an Anti-Cancer Trojan Horse.

Nowakowski A, Drela K, Rozycka J, Janowski M, Lukomska B Stem Cells Dev. 2016; 25(20):1513-1531.

PMID: 27460260 PMC: 5035934. DOI: 10.1089/scd.2016.0120.

Increased CXCL10 expression in MS MSCs and monocytes is unaffected by AHSCT.

Bonechi E, Aldinucci A, Mazzanti B, Di Gioia M, Repice A, Manuelli C Ann Clin Transl Neurol. 2014; 1(9):650-8.

PMID: 25493279 PMC: 4241792. DOI: 10.1002/acn3.92.

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