Morphogenesis and Biomechanics of Engineered Skin Cultured Under Uniaxial Strain

Overview

Journal Adv Wound Care (New Rochelle)

Date 2014 Feb 15

PMID 24527283

Citations 1

Authors

Britani N Blackstone

Heather M Powell

Affiliations

Soon will be listed here.

Abstract

Background: Split-thickness autograft is the standard wound treatment for full-thickness burns. In large burns, sparse availability of uninjured skin prevents rapid closure of the wound, resulting in increased scar tissue formation or mortality. Tissue-engineered skin (ES) offers promise when autografts are not available.

The Problem: ES, constructed from a polymeric scaffold and skin cells, has been shown to reduce donor site area required to permanently close wounds, mortality, and morbidity from scarring but cannot restore all skin functions. Current generations of ES are orders of magnitude weaker than normal human skin, leading to difficulty in surgical application, greater susceptibility to mechanical damage during fabrication and application, and less elasticity and strength once engrafted.

Basic/clinical Science Advances: Previous studies to improve ES biomechanics focus on altering the scaffolding material, which resulted in modest improvements but often inhibited proper skin development. As the skin is naturally under static strain, adding these mechanical cues to the culture environment is hypothesized to improve ES biomechanics. ES was cultured under applied static strains ranging from 0% to 40% strain for a total of 10 days. Strain magnitudes of 10% and 20% strain resulted in significantly stronger ES than unstrained controls, showed upregulation of many genes encoding structural extracellular matrix proteins, and exhibited increased epidermal cell proliferation and differentiation.

Clinical Care Relevance: Enhanced biomechanical properties of ES can allow for facile surgical application and less damage during dressing changes.

Conclusion: These findings suggest that mechanical cues play a significant role in skin development and should be further explored.

Citing Articles

A Combined In Vitro Imaging and Multi-Scale Modeling System for Studying the Role of Cell Matrix Interactions in Cutaneous Wound Healing.

De Jesus A, Aghvami M, Sander E PLoS One. 2016; 11(2):e0148254.

PMID: 26840835 PMC: 4739727. DOI: 10.1371/journal.pone.0148254.

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