Mass Spectrometric Proteomics Reveals That Nuclear Protein Positive Cofactor PC4 Selectively Binds to Cross-linked DNA by a Trans-platinum Anticancer Complex

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2014 Feb 15

PMID 24524683

Citations 13

Authors

Zhifeng Du

Qun Luo

Liping Yang

Tao Bing

Xianchan Li

Wei Guo

Kui Wu

Yao Zhao

Shaoxiang Xiong

Dihua Shangguan

Fuyi Wang

Affiliations

Soon will be listed here.

Abstract

An MS-based proteomic strategy combined with chemically functionalized gold nanoparticles as affinity probes was developed and validated by successful identification and quantification of HMGB1, which is well characterized to interact selectively with 1,2-cross-linked DNA by cisplatin, from whole cell lysates. The subsequent application of this method to identify proteins responding to 1,3-cross-linked DNA by a trans-platinum anticancer complex, trans-PtTz (Tz = thiazole), revealed that the human nuclear protein positive cofactor PC4 selectively binds to the damaged DNA, implying that PC4 may play a role in cellular response to DNA damage by trans-PtTz.

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