Species Identification Through Mitochondrial RRNA Genetic Analysis

Overview

Journal Sci Rep

Specialty Science

Date 2014 Feb 14

PMID 24522485

Citations 48

Authors

Li Yang

Zongqing Tan

Daren Wang

Ling Xue

Min-Xin Guan

Taosheng Huang

Ronghua Li

Affiliations

Soon will be listed here.

Abstract

Inter-species and intraspecific variations in mitochondrial DNA (mtDNA) were observed in a bioinformatics analysis of the mitochondrial genomic sequences of 11 animal species. Some highly conserved regions were identified in the mitochondrial 12S and 16S ribosomal RNA (rRNA) genes of these species. To test whether these sequences are universally conserved, primers were designed to target the conserved regions of these two genes and were used to amplify DNA from 21 animal tissues, including two of unknown origin. By sequencing these PCR amplicons and aligning the sequences to a database of non-redundant nucleotide sequences, it was confirmed that these amplicons aligned specifically to mtDNA sequences from the expected species of origin. This molecular technique, when combined with bioinformatics, provides a reliable method for the taxonomic classification of animal tissues.

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