Structural Bases for the Interaction and Stabilization of the Human Amino Acid Transporter LAT2 with Its Ancillary Protein 4F2hc

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Feb 12

PMID 24516142

Citations 48

Authors

Albert Rosell

Marcel Meury

Elena Alvarez-Marimon

Meritxell Costa

Laura Perez-Cano

Antonio Zorzano

Juan Fernandez-Recio

Manuel Palacin

Dimitrios Fotiadis

Affiliations

Soon will be listed here.

Abstract

Heteromeric amino acid transporters (HATs) are the unique example, known in all kingdoms of life, of solute transporters composed of two subunits linked by a conserved disulfide bridge. In metazoans, the heavy subunit is responsible for the trafficking of the heterodimer to the plasma membrane, and the light subunit is the transporter. HATs are involved in human pathologies such as amino acidurias, tumor growth and invasion, viral infection and cocaine addiction. However structural information about interactions between the heavy and light subunits of HATs is scarce. In this work, transmission electron microscopy and single-particle analysis of purified human 4F2hc/L-type amino acid transporter 2 (LAT2) heterodimers overexpressed in the yeast Pichia pastoris, together with docking analysis and crosslinking experiments, reveal that the extracellular domain of 4F2hc interacts with LAT2, almost completely covering the extracellular face of the transporter. 4F2hc increases the stability of the light subunit LAT2 in detergent-solubilized Pichia membranes, allowing functional reconstitution of the heterodimer into proteoliposomes. Moreover, the extracellular domain of 4F2hc suffices to stabilize solubilized LAT2. The interaction of 4F2hc with LAT2 gives insights into the structural bases for light subunit recognition and the stabilizing role of the ancillary protein in HATs.

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