Effect of Beta-carotene on Titanium Oxide Nanoparticles-induced Testicular Toxicity in Mice

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2014 Feb 12

PMID 24515782

Citations 24

Authors

Mahmoud Orazizadeh

Layasadat Khorsandi

Forouzan Absalan

Mahmoud Hashemitabar

Erfan Daneshi

Affiliations

Soon will be listed here.

Abstract

Objective: This study evaluated the protective effect of beta-carotene (BC) on titanium oxide nanoparticle (TNP) induced spermatogenesis defects in mice.

Materials And Methods: Thirty-two NMRI mice were randomly divided into four groups. BC group received 10 mg/kg of BC for 35 days. TNP group received 300 mg/kg TNP for 35 days. TNP+BC group initially received 10 mg/kg BC for 10 days and was followed by concomitant administration of 300 mg/kg TNP for 35 days. Control group received only normal saline for 35 days. Epididymal sperm parameters, testicular histopathology, spermatogenesis assessments and testosterone assay were performed for evaluation of the TNP and BC effects on testis.

Results: Serum testosterone levels were markedly decreased in TNP-intoxicated mice. Epididymal sperm parameters including sperm number, motility and percentage of abnormality were significantly changed in TNP-intoxicated mice (p < 0.01). Histopathological criteria such as epithelial vacuolization, sloughing of germ cells and detachment were significantly increased in TNP-intoxicated mice (p < 0.001). BC+TNP treatment significantly prevented these changes (p < 0.05). BC also significantly elevates testosterone levels in BC+TNP group compared to TNP-treated mice (p < 0.01).

Discussion And Conclusion: The results of this study demonstrated that BC improved the spermatogenesis defects in TNP-treated mice. BC had a potent protective effect against the testicular toxicity and might be clinically useful.

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Preventive effects of quercetin against inflammation and apoptosis in cyclophosphamide-induced testicular damage.

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Abouzaripour M, Daneshi E, Amiri F, Naseri S, Allahveisi A Avicenna J Phytomed. 2023; 13(5):541-550.

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Toxic effects of titanium dioxide nanoparticles on reproduction in mammals.

Minghui F, Ran S, Yuxue J, Minjia S Front Bioeng Biotechnol. 2023; 11:1183592.

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