A Cell-free Approach to Accelerate the Study of Protein-protein Interactions in Vitro

Overview

Journal Interface Focus

Specialty Biology

Date 2014 Feb 11

PMID 24511386

Citations 22

Authors

E Sierecki

N Giles

M Polinkovsky

M Moustaqil

K Alexandrov

Y Gambin

Affiliations

Soon will be listed here.

Abstract

Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.

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