Genetic Manipulation of Staphylococcus Aureus

Overview

Journal Curr Protoc Microbiol

Specialty Microbiology

Date 2014 Feb 11

PMID 24510849

Citations 23

Authors

Olaf Schneewind

Dominique Missiakas

Affiliations

Soon will be listed here.

Abstract

Staphylococcus aureus is a facultative anaerobic Gram-positive coccus and a member of the normal skin flora, as well as that of the nasal passages of humans. However, S. aureus can also gain entry into the host and cause life-threatening infections or persist as disease foci that develop into suppurative abscesses. While genetically tractable, the manipulation of S. aureus remains challenging. This unit describes methods developed in our laboratory for gene disruption by allelic replacement and transposition. We also provide a protocol for bacteriophage-mediated transduction of mutants marked with selectable alleles and describe plasmid utilization for complementation studies.

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