A Synthetic Luciferin Improves Bioluminescence Imaging in Live Mice

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2014 Feb 11

PMID 24509630

Citations 84

Authors

Melanie S Evans

Joanna P Chaurette

Spencer T Adams Jr

Gadarla R Reddy

Miranda A Paley

Neil Aronin

Jennifer A Prescher

Stephen C Miller

Affiliations

Soon will be listed here.

Abstract

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.

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