Isolation and Molecular Characterization of Biofouling Bacteria and Profiling of Quorum Sensing Signal Molecules from Membrane Bioreactor Activated Sludge

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2014 Feb 7

PMID 24499972

Citations 16

Authors

Harshad Lade

Diby Paul

Ji Hyang Kweon

Affiliations

Soon will be listed here.

Abstract

The formation of biofilm in a membrane bioreactor depends on the production of various signaling molecules like N-acyl homoserine lactones (AHLs). In the present study, a total of 200 bacterial strains were isolated from membrane bioreactor activated sludge and screened for AHLs production using two biosensor systems, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136. A correlation between AHLs production and biofilm formation has been made among screened AHLs producing strains. The 16S rRNA gene sequence analysis revealed the dominance of Aeromonas and Enterobacter sp. in AHLs production; however few a species of Serratia, Leclercia, Pseudomonas, Klebsiella, Raoultella and Citrobacter were also identified. The chromatographic characterization of sludge extract showed the presence of a broad range of quorum sensing signal molecules. Further identification of sludge AHLs by thin layer chromatography bioassay and high performance liquid chromatography confirms the presence of C4-HSL, C6-HSL, C8-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C12-HSL and C14-HSL. The occurrence of AHLs in sludge extract and dominance of Aeromonas and Enterobacter sp. in activated sludge suggests the key role of these bacterial strains in AHLs production and thereby membrane fouling.

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References

Chen J, Koh C, Sam C, Yin W, Chan K . Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9. Sensors (Basel). 2013; 13(10):13217-27. PMC: 3859060. DOI: 10.3390/s131013217. View

OToole G, Kolter R . Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28(3):449-61. DOI: 10.1046/j.1365-2958.1998.00797.x. View

Anbazhagan D, Mansor M, Yan G, Md Yusof M, Hassan H, Sekaran S . Detection of quorum sensing signal molecules and identification of an autoinducer synthase gene among biofilm forming clinical isolates of Acinetobacter spp. PLoS One. 2012; 7(7):e36696. PMC: 3398020. DOI: 10.1371/journal.pone.0036696. View

Chong G, Kimyon O, Rice S, Kjelleberg S, Manefield M . The presence and role of bacterial quorum sensing in activated sludge. Microb Biotechnol. 2012; 5(5):621-33. PMC: 3815874. DOI: 10.1111/j.1751-7915.2012.00348.x. View

Daniels R, Vanderleyden J, Michiels J . Quorum sensing and swarming migration in bacteria. FEMS Microbiol Rev. 2004; 28(3):261-89. DOI: 10.1016/j.femsre.2003.09.004. View

Johnson L . Microcolony and biofilm formation as a survival strategy for bacteria. J Theor Biol. 2007; 251(1):24-34. DOI: 10.1016/j.jtbi.2007.10.039. View

Ravn L, Christensen A, Molin S, Givskov M, Gram L . Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics. J Microbiol Methods. 2001; 44(3):239-51. DOI: 10.1016/s0167-7012(01)00217-2. View

Wagner M, Loy A . Bacterial community composition and function in sewage treatment systems. Curr Opin Biotechnol. 2002; 13(3):218-27. DOI: 10.1016/s0958-1669(02)00315-4. View

Dong Y, Zhang X, An S, Xu J, Zhang L . A novel two-component system BqsS-BqsR modulates quorum sensing-dependent biofilm decay in Pseudomonas aeruginosa. Commun Integr Biol. 2009; 1(1):88-96. PMC: 2633808. DOI: 10.4161/cib.1.1.6717. View

10.

Hugenholtz P, Goebel B, Pace N . Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol. 1998; 180(18):4765-74. PMC: 107498. DOI: 10.1128/JB.180.18.4765-4774.1998. View

11.

Kim S, Oh H, Jo S, Yeon K, Lee C, Lim D . Biofouling control with bead-entrapped quorum quenching bacteria in membrane bioreactors: physical and biological effects. Environ Sci Technol. 2012; 47(2):836-42. DOI: 10.1021/es303995s. View

12.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

13.

Biswa P, Doble M . Production of acylated homoserine lactone by gram-positive bacteria isolated from marine water. FEMS Microbiol Lett. 2013; 343(1):34-41. DOI: 10.1111/1574-6968.12123. View

14.

Wang H, Cai T, Weng M, Zhou J, Cao H, Zhong Z . Conditional production of acyl-homoserine lactone-type quorum-sensing signals in clinical isolates of enterobacteria. J Med Microbiol. 2006; 55(Pt 12):1751-1753. DOI: 10.1099/jmm.0.46756-0. View

15.

Kumari A, Pasini P, Daunert S . Detection of bacterial quorum sensing N-acyl homoserine lactones in clinical samples. Anal Bioanal Chem. 2008; 391(5):1619-27. DOI: 10.1007/s00216-008-2002-3. View

16.

Stoodley P, Wilson S, Hall-Stoodley L, Boyle J, Lappin-Scott H, Costerton J . Growth and detachment of cell clusters from mature mixed-species biofilms. Appl Environ Microbiol. 2001; 67(12):5608-13. PMC: 93350. DOI: 10.1128/AEM.67.12.5608-5613.2001. View

17.

Rinaudi L, Giordano W . An integrated view of biofilm formation in rhizobia. FEMS Microbiol Lett. 2009; 304(1):1-11. DOI: 10.1111/j.1574-6968.2009.01840.x. View

18.

Yeon K, Cheong W, Oh H, Lee W, Hwang B, Lee C . Quorum sensing: a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment. Environ Sci Technol. 2009; 43(2):380-5. DOI: 10.1021/es8019275. View

19.

Rasmussen T, Bjarnsholt T, Skindersoe M, Hentzer M, Kristoffersen P, Kote M . Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol. 2005; 187(5):1799-814. PMC: 1063990. DOI: 10.1128/JB.187.5.1799-1814.2005. View

20.

Miura Y, Watanabe Y, Okabe S . Membrane biofouling in pilot-scale membrane bioreactors (MBRs) treating municipal wastewater: impact of biofilm formation. Environ Sci Technol. 2007; 41(2):632-8. DOI: 10.1021/es0615371. View