Small-molecule Probes Elucidate Global Enzyme Activity in a Proteomic Context

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2014 Feb 7

PMID 24499666

Citations 4

Authors

Jun-Seok Lee

Young-Hwa Yoo

Chang No Yoon

Affiliations

Soon will be listed here.

Abstract

The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of small-molecule probes in proteomics studies and highlight some recent examples in which this principle has been applied.

Citing Articles

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