Inhibition of Chicken Calpain II by Proteins of the Cystatin Superfamily and Alpha 2-macroglobulin

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Dec 1

PMID 2449169

Citations 1

Authors

C Crawford

Affiliations

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Abstract

Inhibition of chicken calpain II by proteins of the cystatin superfamily and alpha 2-macroglobulin was investigated. Human liver cystatins A and B, human cystatin C, chicken cystatin and rat T-kininogen were found not to be inhibitory. Inhibition was, however, observed for bovine and rat kininogens, with Ki (inhibition constant) values of 0.8 nM and 30 nM respectively. alpha 2-Macroglobulin inhibits calpain with an initial rate constant of the order of 3 X 10(4) M-1.S-1. Calpain complexed with alpha 2-macroglobulin showed only limited reactivity towards azocasein, but reacted readily with the peptide substrate Suc-Leu-Tyr-4-methyl-7-coumarylamide and with L-3-carboxy-trans-2,3-epoxypropionyl-leucylamido-(4-guanidin o)butane (E-64). The calpain in the complexes was at least partially protected from loss of activity due to autolysis. The calpain-alpha 2-macroglobulin complexes contained both the calpain subunits.

Citing Articles

Kinetics of inhibition of platelet calpain II by human kininogens.

Bradford H, Schmaier A, Colman R Biochem J. 1990; 270(1):83-90.

PMID: 2396995 PMC: 1131681. DOI: 10.1042/bj2700083.

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