Antigenic Probes Locate a Serum-gelsolin-interaction Site on the C-terminal Part of Actin

Overview

Journal Biochem J

Specialty Biochemistry

Date 1987 Dec 1

PMID 2449166

Citations 8

Authors

M Boyer

J Feinberg

H K Hue

J P Capony

Y Benyamin

C Roustan

Affiliations

Soon will be listed here.

Abstract

The implication of part of the C-terminal of actin (within the 285-375 sequence) in the interaction of serum gelsolin was investigated by the use of specific antibodies. These antibodies were directed against two or three discrete epitopes, one of which was specific for skeletal-muscle actin. Some of these epitopes were found to be near the serum gelsolin-actin interface. Thus it can be assumed that part of the C-terminal of actin is exposed at the barbed end of the actin filament. The interaction between tropomyosin and actin was also studied.

Citing Articles

Interaction of skeletal-muscle myosin subfragment-1 with the actin-(338-348) peptide.

Labbe J, Lelievre S, Boyer M, Benyamin Y Biochem J. 1994; 299 ( Pt 3):875-9.

PMID: 8192679 PMC: 1138102. DOI: 10.1042/bj2990875.

Acanthamoeba actin and profilin can be cross-linked between glutamic acid 364 of actin and lysine 115 of profilin.

Vandekerckhove J, Kaiser D, Pollard T J Cell Biol. 1989; 109(2):619-26.

PMID: 2569469 PMC: 2115722. DOI: 10.1083/jcb.109.2.619.

Antigenic probes locate binding sites for the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, aldolase and phosphofructokinase on the actin monomer in microfilaments.

Mejean C, Pons F, Benyamin Y, Roustan C Biochem J. 1989; 264(3):671-7.

PMID: 2482731 PMC: 1133639. DOI: 10.1042/bj2640671.

Comparative study of invertebrate actins: antigenic cross-reactivity versus sequence variability.

Hue H, Benyamin Y, Roustan C J Muscle Res Cell Motil. 1989; 10(2):135-42.

PMID: 2474567 DOI: 10.1007/BF01739969.

Characterization of an actin-myosin head interface in the 40-113 region of actin using specific antibodies as probes.

Labbe J, Mejean C, Benyamin Y, Roustan C Biochem J. 1990; 271(2):407-13.

PMID: 2146951 PMC: 1149569. DOI: 10.1042/bj2710407.

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