Evidence for Alterations in Stimulatory G Proteins and Oxytocin Levels in Children with Autism

Overview

Journal Psychoneuroendocrinology

Specialties Endocrinology
Neurology
Psychiatry

Date 2014 Feb 4

PMID 24485488

Citations 22

Authors

Jill D Jacobson

Kathryn A Ellerbeck

Kelsie A Kelly

Kandace K Fleming

T Rene Jamison

Charles W Coffey

Catherine M Smith

R Matthew Reese

Scott A Sands

Affiliations

Soon will be listed here.

Abstract

The neurotransmitter oxytocin plays an important role in social affiliation. Low oxytocin levels and defects in the oxytocin receptor have been reported in childhood autism. However, little is known about oxytocin's post-receptor signaling pathways in autism. Oxytocin signals via stimulatory and inhibitory G proteins. c-fos mRNA expression has been used as a marker of OT signaling as well as of G protein signaling. Herein, we hypothesized that oxytocin and its signaling pathways would be altered in children with autism. We measured plasma oxytocin levels by ELISA, G-protein and c-fos mRNA by PCR, and G proteins by immunoblot in cultured peripheral blood mononuclear cells (PBMCs) in children with autism and in age-matched controls. Males with autism displayed elevated oxytocin levels compared to controls (p<0.05). Children with autism displayed significantly higher mRNA for stimulatory G proteins compared to controls (p<0.05). Oxytocin levels correlated strongly positively with c-fos mRNA levels, but only in control participants (p<0.01). Oxytocin, G-protein, and c-fos mRNA levels correlated inversely with measures of social and emotional behaviors, but only in control participants. These data suggest that children with autism may exhibit a dysregulation in oxytocin and/or its signaling pathways.

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