Conventional Transmission Electron Microscopy

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2014 Feb 1

PMID 24482357

Citations 64

Authors

Mark Winey

Janet B Meehl

Eileen T OToole

Thomas H Giddings Jr

Affiliations

Soon will be listed here.

Abstract

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.

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