Structure and Expression of the Human Thymocyte Antigens CD1a, CD1b, and CD1c

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Dec 1

PMID 2447586

Citations 39

Authors

L H MARTIN

F Calabi

F A Lefebvre

C A Bilsland

C Milstein

Affiliations

Soon will be listed here.

Abstract

The CD1 human antigens are a family of at least three components, CD1a, CD1b, and CD1c, that are characteristic of the cortical stage of thymocyte maturation. CD1a was originally named HTA1 or T6 and thought to be the human equivalent of mouse Tla. The genes coding for all three have now been identified by transfection into mouse cells. The transfectants express the surface antigens that can then be recognized by the corresponding cluster of monoclonal antibodies used to define the three members of CD1. The full sequence of the genomic DNA is described for all three. The intron-exon structure of CD1a is deduced by comparison with a near-full-length cDNA clone. Similar structures are proposed for the other two, largely based on sequence homology. An unusually long 5'-untranslated exon (280 bases long) is highly conserved between the three genes, suggesting an important but unknown function. CD1c has a duplicated form of this exon that is thought to be spliced out. The major homology between the three antigens is in the beta 2-microglobulin-binding domain. The general relatedness to major histocompatibility complex class I and class II molecules is significant but low, with no section of higher homology to mouse Tla.

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References

von Heijne G . Signal sequences. The limits of variation. J Mol Biol. 1985; 184(1):99-105. DOI: 10.1016/0022-2836(85)90046-4. View

Reinherz E, Kung P, Goldstein G, Levey R, Schlossman S . Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-92. PMC: 348542. DOI: 10.1073/pnas.77.3.1588. View

Mulligan R, Berg P . Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1981; 78(4):2072-6. PMC: 319285. DOI: 10.1073/pnas.78.4.2072. View

Ziegler A, Milstein C . A small polypeptide different from beta2-microglobin associated with a human cell surface antigen. Nature. 1979; 279(5710):243-4. DOI: 10.1038/279243a0. View

Neuberger M . Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells. EMBO J. 1983; 2(8):1373-8. PMC: 555285. DOI: 10.1002/j.1460-2075.1983.tb01594.x. View

Staden R . An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982; 10(9):2951-61. PMC: 320667. DOI: 10.1093/nar/10.9.2951. View

Mount S . A catalogue of splice junction sequences. Nucleic Acids Res. 1982; 10(2):459-72. PMC: 326150. DOI: 10.1093/nar/10.2.459. View

MARTIN L, Calabi F, Milstein C . Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens. Proc Natl Acad Sci U S A. 1986; 83(23):9154-8. PMC: 387093. DOI: 10.1073/pnas.83.23.9154. View

Amiot M, Bernard A, Raynal B, Knapp W, Deschildre C, Boumsell L . Heterogeneity of the first cluster of differentiation: characterization and epitopic mapping of three CD1 molecules on normal human thymus cells. J Immunol. 1986; 136(5):1752-8. View

10.

van de Rijn M, Lerch P, Knowles R, Terhorst C . The thymic differentiation markers T6 and M241 are two unusual MHC class I antigens. J Immunol. 1983; 131(2):851-5. View

11.

Knowles R, Bodmer W . A monoclonal antibody recognizing a human thymus leukemia-like antigen associated with beta 2-microglobulin. Eur J Immunol. 1982; 12(8):676-81. DOI: 10.1002/eji.1830120810. View

12.

Vega M, Bragado R, Ezquerra A, de Castro J . Variability and conformation of HLA class I antigens: a predictive approach to the spatial arrangement of polymorphic regions. Biochemistry. 1984; 23(5):823-31. DOI: 10.1021/bi00300a007. View

13.

Potter H, Weir L, Leder P . Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci U S A. 1984; 81(22):7161-5. PMC: 392097. DOI: 10.1073/pnas.81.22.7161. View

14.

Srivastava R, Duceman B, Biro P, Sood A, Weissman S . Molecular organization of the class I genes of human major histocompatibility complex. Immunol Rev. 1985; 84:93-121. DOI: 10.1111/j.1600-065x.1985.tb01127.x. View

15.

Bernabeu C, van de Rijn M, Lerch P, terHorst C . Beta 2-microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature. 1984; 308(5960):642-5. DOI: 10.1038/308642a0. View

16.

Kefford R, Calabi F, Fearnley I, Burrone O, Milstein C . Serum beta 2-microglobulin binds to a T-cell differentiation antigen and increases its expression. Nature. 1984; 308(5960):641-2. DOI: 10.1038/308641a0. View

17.

Staden R . The current status and portability of our sequence handling software. Nucleic Acids Res. 1986; 14(1):217-31. PMC: 339382. DOI: 10.1093/nar/14.1.217. View

18.

Olive D, Dubreuil P, Mawas C . Two distinct TL-like molecular subsets defined by monoclonal antibodies on the surface of human thymocytes with different expression on leukemia lines. Immunogenetics. 1984; 20(3):253-64. DOI: 10.1007/BF00364207. View

19.

Kozak M . Bifunctional messenger RNAs in eukaryotes. Cell. 1986; 47(4):481-3. DOI: 10.1016/0092-8674(86)90609-4. View

20.

Calabi F, Milstein C . A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6. Nature. 1986; 323(6088):540-3. DOI: 10.1038/323540a0. View