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Organization of the Human Fetal Subpallium

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Journal Front Neuroanat
Date 2014 Jan 30
PMID 24474906
Citations 15
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Abstract

The subpallium comprises large parts of the basal ganglia including striatum and globus pallidus. Genes and factors involved in the development of the subpallium have been extensively studied in most vertebrates, including amphibians, birds, and rodents. However, our knowledge on patterning of the human subpallium remains insufficient. Using double fluorescent immunohistochemistry, we investigated the protein distribution of transcription factors involved in patterning of the subventricular zone (SVZ) in the human forebrain at late embryonic development. Furthermore, we compared the development of cortical and striatal precursors between human fetal brain and E14 and E16 fetal rat brains. Our results reveal that DLX2 marks SVZ precursors in the entire subpallium. Individual subpallial subdomains can be identified based on co-expression of DLX2 with either PAX6 or NKX2-1. SVZ precursors in the dorsal LGE and preopto-hypothalamic boundary are characterized by DLX2/PAX6 co-expression, while precursors in the MGE and preoptic region co-express DLX2/NKX2-1. SVZ precursors in the ventral LGE are DLX2(+)/PAX6(-)/NKX2-1(-). In terms of staging comparisons, the development of the corpus striatum in the human fetal brain during late embryonic stages corresponds well with the development of the striatum observed in E14 fetal rat brains. Our study demonstrates that the pattern underlying the development of the subpallium is highly conserved between rodents and humans and suggests a similar function for these factors in human brain development. Moreover, our data directly influence the application of ganglionic eminence derived human tissue for cell therapeutic approaches in neurodegenerative disorders such as Huntington's disease.

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References
1.
Terzic J, Saraga-Babic M . Expression pattern of PAX3 and PAX6 genes during human embryogenesis. Int J Dev Biol. 1999; 43(6):501-8. View

2.
Schackel S, Pauly M, Piroth T, Nikkhah G, Dobrossy M . Donor age dependent graft development and recovery in a rat model of Huntington's disease: histological and behavioral analysis. Behav Brain Res. 2013; 256:56-63. DOI: 10.1016/j.bbr.2013.07.053. View

3.
Simeone A, Acampora D, Mallamaci A, Stornaiuolo A, DApice M, Nigro V . A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo. EMBO J. 1993; 12(7):2735-47. PMC: 413524. DOI: 10.1002/j.1460-2075.1993.tb05935.x. View

4.
Moreno N, Gonzalez A, Retaux S . Evidences for tangential migrations in Xenopus telencephalon: developmental patterns and cell tracking experiments. Dev Neurobiol. 2008; 68(4):504-20. DOI: 10.1002/dneu.20603. View

5.
Bulfone A, Kim H, Puelles L, Porteus M, Grippo J, Rubenstein J . The mouse Dlx-2 (Tes-1) gene is expressed in spatially restricted domains of the forebrain, face and limbs in midgestation mouse embryos. Mech Dev. 1993; 40(3):129-40. DOI: 10.1016/0925-4773(93)90071-5. View