[Identification and Localisation of Tryptophan Synthetase in Seeds of Juglans Regia L]
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Abstract
Cell-free extracts from seeds of Juglans regia synthesize tryptophan from L-serine and indole. Tryptophan synthetase has maximal activity in the range between pH 7 and 8. The enzyme is associated with a particulate fraction (density 1,210 g/ml) which is separated from the mitochondria (density 1,191 g/ml) after isopycnic density centrifugation on a continuous sucrose gradient.
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PMID: 24442196 DOI: 10.1007/BF00390500.
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